Heparin sodium injection 1000 units

For children, heparin sodium is administered by intermittent IV infusion in a range of 60 to 80 units/kg of body weight every four hour. For a 57-lb child, calculate the range, in mL, of a heparin sodium injection containing 5000 units/mL to be administered daily. 

Injection 1,000 and 2,000 units Rx) Heparin Sodium and 0.9% Sodium Chloride (Clintec)... 

PRP and PPP preparation. If rats or mice are used, they are anaesthetized by an i.p. injection of sodium pentobarbital (50 mg/Kg), and blood is drawn into heparin (5 units/ml final) or citrate/dextrose (final concentration 0.38% and 0.48% (wt/vol), respectively) anticoagulant from the dorsal aorta or by heart puncture, with a 22-gauge needle (the choice of heparin as anticoagulant appears to be more appropriate, because chelation of divalent cations by trisodium citrate or EDTA might affect platelet-tumor cell interaction). Blood is then diluted with an equal volume of 0.9% NaCl, and centrifuged for 7 min at 400 x g on preformed gradients of 70% Percoll containing 0.9% NaCl. The yellowish supernatant is the PPP, while PRP forms a white band between PPP and the percoll layer. Platelets in PRP are counted with a Coulter counter and diluted with PPP to a concentration of 10 /ml. PPP is a source of prothrombin, and should thus be included in the assay. 

In humans, contact with LPS may occur not only during a bacterial infection but also via LPS-contaminated medicaments and solutions administrated intravenously (parenteralia). Since the biological effects of LPS may appear even at concentrations of 1 ng per 1 kg of body weight, drugs intended for parenteral use, have to be endotoxin-free , i.e., thoroughly depyrogenated. In fact parenteralia have to comply with LPS threshold limits (in EU , endotoxin unit) regulated by pharmacopoeias. For example, tetracycline hydrochloride may not contain more than 0.5 ELf/mg. Similar limits exist for insulin (0.8 EU/insulin unit), hyaluronidase (2.3 EU/ hyaluronidase unit), the sodium salt ofheparin for injection (0.003 EU/heparin unit). ... 

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