Insulin units

Then, 1.3 ml of glycerine are mixed with 0.5 ml of a 25% solution of methyl p-hydroxy-benzoate in ethanol, and 50 ml of distilled water are added. To the produced mixture are, after sterile filtration, added 10 ml of the stock solution 1, 2.5 ml of the stock solution 2 and 10 ml of the stock solution 3, after which 3.0 ml of sterile 0.1 N sodium hydroxide are added, and the mixture is filled up with sterile distilled water to a volume of 100 ml. The insulin will be precipitated amorphously by the admixture of the sodium hydroxide, and the produced suspension acquires the pH value of 7. It will contain approximately 1 gamma zinc per insulin unit. 

Q17 A patient is receiving continuous insulin aspart 0.05 unit/mL in 0.9% sodium chloride at a rate of 5 mL/min. The amount of insulin units that the patient receives in 1 hour is ... 

Conversion of Pramlintide Dose to Insulin Unit Equivalents ... 

Isophane insulin is a white suspension of rod-shaped crystals approximately 30- Jm long, and free from large aggregates of crystals after being subjected to moderate agitation. It contains either 1.4-1.8% glycerol, 0.15-0.17% meta-cresol, and 0.06-0.07% phenol on a wt/vol basis, or 1.4—1.8% glycerol and 0.20-0.25% phenol (wt/vol), at a pH of 7.1-7.4. It also contains 0.15-0.25% (wt/vol) of sodium phosphate, 0.01-0.04 mg of zinc, and 0.3—0.6 mg of protamine for each USP insulin unit. The insoluble matter in the suspension is crystalline and contains not more than traces of amorphous material. 

Insulin Zinc Suspension. Lente Insulin Lente lie-tin Insulin Novo Lente Human Monotard Insulin Ultra -card Lentard Monotard Semitard Ultratard. Sterile suspension, in a buffered water medium, of insulin modified by the addition of zinc chloride in a manner such that the solid phase of the suspension consists of a mixture of crystals and amorphous materia] in a ratio of approx 7 3 Hallas -Moller, Lancet 267, 1029 (1954). Contains 40 Or 80 U.S.P, insulin units/ml. Prepn of amorphous zinc insulin suspension Petersen et aL, U.S. pat, 2 82,203 (1959 to Novo Terapeu-tisk Lab. A IS). 

Note Insulin Zinc Suspension Prompt Insulin Zinc Suspension (Amorphous) Semilente Iletin and Insulin Zinc Suspension Extended Insulin Zinc Suspension (Crystalline) Vltrutense Iletin are suspensions of minute particles of zinc insulin and differ only in the particle size which determines the duration of their action. Contain 40 or 80 U.S.P. insulin units/ ml. 

It is available as a sterile, acidified or neutral solution of insulin. The solution has a potency of 40, 80, 100 or 500 USP Insulin Units in each ml. 

Each mL is prepared from enough insulin to provide either 40, 80, or 100 USP Insulin units of insulin activity. 

It is invariably obtained as a sterile suspension of insulin in buffered water for injection, carefully modified by the addition of zinc chloride (ZnCl2) in such a maimer that the solid-phase of the suspension comprises of a mixture of crystalline as well as amorphous insulin present approximately in a ratio of 7 portions of crystals and 3 portions of amorphous substance. Each mL is obtained from sufficient insulin to provide either 40, 80, or 100 USP Insulin Units of the Insulin Activity. 

Instruct the client to dial in the number of insulin units needed to inject. 

M. Bodanszky, J. Fried, Process for preparing human insulin. United States Patent 3276961,1966... 

Brange, J., 1977, Improvements in or relating to biphasic insulin preparations comprising crystalline insulin and monodesamido insulin, United Kingdom Patent 1560232. 

In humans, contact with LPS may occur not only during a bacterial infection but also via LPS-contaminated medicaments and solutions administrated intravenously (parenteralia). Since the biological effects of LPS may appear even at concentrations of 1 ng per 1 kg of body weight, drugs intended for parenteral use, have to be endotoxin-free , i.e., thoroughly depyrogenated. In fact parenteralia have to comply with LPS threshold limits (in EU , endotoxin unit) regulated by pharmacopoeias. For example, tetracycline hydrochloride may not contain more than 0.5 ELf/mg. Similar limits exist for insulin (0.8 EU/insulin unit), hyaluronidase (2.3 EU/ hyaluronidase unit), the sodium salt ofheparin for injection (0.003 EU/heparin unit). ... 

The Insulin Unit.—This was defined originally as the amount required to evoke hypoglycsemic convulsions in a fasting rabbit of 2 kg. weight. Wide variations in animal sensitivity have led to the abandonment of this unit in favour of one based on the use of a dry, soluble insulin hydrochloride preparation kept in the National Institute for Medical Research, in London. One insulin unit equals 0-125 mg. of this material. 

---