Syringe Hamilton

FIGURE 5.7 Eiquipment for manual application, e.g., 500-pl Hamilton syringe (lower), Pasteur pipette (middle), and 2-ml measuring pipette (upper). 

Some equipment for manual applieation is shown in Figure 5.7. A Hamilton syringe of 500 pi, or a measuring or volumetric pipette of, say, 1 or 2 ml, with Peleus ball, or a Pasteur pipette with an aspirating bulb can be employed. 

Fig. 8.5 Capsaicin accumulates in blisters/vesicles on surface of placenta, left panel) Total ion chromatogram of oil in habanero vesicle. The oil in the vesicle was collected directly with a Hamilton syringe, diluted with hexane, and analyzed on a varian GC-MS, DB-5 column. The capsaicin peak was identified based on match with NIST MS library, right upper panel) A stereoscope view of habanero placenta (5x magnification) and seeds are visible upper left panel). Arrows indicate blisters, right lower panel) A scanning electron micrograph of habanero placenta (40 X magnification). A color version of the image is on line...

Add the samples with a syringe (e.g., Hamilton syringe) or special pipette tips to form a lower layer below the electrophoresis buffer within the slots. 

Figure 2-13 Hamilton syringe with a volume of 1 jjlL and divisions of 0.02 xL on the glass barrel. [Courtesy Hamilton Co., Reno, NV.]...

Figure 11-6a shows experimental results for the manual titration of a hexaprotic weak acid, H A, with NaOH. Because the compound is difficult to purify, only a tiny amount was available for titration. Just 1.430 mg was dissolved in 1.00 mL of water and titrated with microliter quantities of 0.065 92 M NaOH, delivered with a Hamilton syringe. 

Use a Hamilton syringe to inject 1 to 50 jul LOX supernatant solution, making sure the tip of the syringe reaches the main part of the sample chamber. 

Perhaps the simplest solvent dispersion method is that developed by Batzri and Korn (1973). Phospholipids and other lipids to be a part of the liposomal membrane are first dissolved in ethanol. This ethanolic solution is then rapidly injected into an aqueous solution of 0.16 M KC1 using a Hamilton syringe, resulting in a maximum concentration of no more than 7.5% ethanol. Using this method, single bilayer liposomes of about 25-nm diameter can be created that are indistinguishable from those formed by mechanical sonication techniques. The main disadvantages of ethanolic injection are the limited solubility of some lipids in the solvent (about 40 mM for phosphatidyl choline) and the dilute nature of the resultant liposome suspension. However, for the preparation of small quantities of SUVs, this method may be one of the best available. 

Luciferin (3 xL of 26.5 mg/mL luciferin solution in NaCl 0.9%) is administered intracerebro-ventricularly (i.c.v.) to anesthetize animals. The injection is carried out according to specific stereotaxic coordinates (bregma, —0.25 mm lateral, 1 mm depth, 2.25 mm) by the use of a Hamilton syringe rotated on the coronal plate about 3° from the orthogonal position. 

Special Supplies 1. Gavage needles 20 or 22 G with 1.25 or 2.25 mm ball, for small or large (>35 g) mice, respectively 2. Hamilton syringes 250, 100, and 10 xl with needles 3. Wire platforms for animal cage bottoms 4. 35 ml glass centrifuge tubes with screw caps 5. Ceramic mortar and pestle (two or more)... 

The cholestane and taurocholate serve as internal standards. They should be dispensed with a Hamilton syringe or other positive displacement device to insure accurate delivery to each sample. Let solvents evaporate before proceeding. The exact amount of taurocholate used should also be dispensed into a scintillation vial to accurately determine the number of dpm added to each sample. 

The experimental set-up used Hamilton syringes for liquid feed [114]. Two syringes pump and another two extract solution from the mixer. The pressure drop was measured differentially, by determining the pressure at one inlet and outlet, respectively. 

In order to ensure accuracy of vector delivery to the heart and to prevent any potential injury to the animal from any movements during vector administration, neonates are anesthetized by induced hypothermia in which the animals are wrapped in cellophane and floated in an ice water bath until their activity slows. The neonate will be removed from ice and placed on a pad under a microscope and fight, and rubbed down with a sterile alcohol pad. Up to 25 ul of vector can be directly injected into the heart through the chest cavity using a 33-gauge needle on a Hamilton syringe. 

Inject, using a Hamilton syringe (total volume of the syringe 5 iL), a fivefold dilution first for evaluating the intensity of the mass signals. If necessary, inject the rest of the sample. 

Hamilton syringes of 1-, 10-, 25-, or 50-yL capacity were used. Standards were prepared in 2-mL vials sealed with Teflon-lined silicon rubber septa and crimped with aluminum serum caps. 

The charcoal tube samples were desorbed with 2 ml. of chrcanatoquality carbon disulfide (CS2), dispensed with an automatic pipette, in vials with Teflon-lined septum caps. Vials were agitated for 30 minutes on an SKC developing vibrator equipped with an automatic timer. Standards and spiked charcoal tubes with known amounts of solvent were prepared with Hamilton syringes. A Varian Model 1800 Gas Chromatograph equipped with a flame ionization detector was used to analyze the CS2-desorbed samples. Two GC colums were used ... 

The metal complexation of ampholytes may cause unwanted results when iron-containing tools are used for handling the sample (e.g., syringes with metal needles, metal tubing, etc.). This was demonstrated with transferrin, a metalbinding protein, when iron-complexed forms of transferrin appeared in the mobilization pattern of an iron-free sample upon mixing the ampholytes and the protein solution with a Hamilton syringe equipped with metal needle [36]. 

Titration of the resulting organolithium solution withdraw by means of a Hamilton syringe an aliquot (1 mL) of the organolithium solution and hydrolyse it in 20 mL of distilled water contained in a 50 mL conical flask. Titration with standard HCI using 3 drops of an ethanolic phenolphthalein solution as an indicator gives the total alkali concentration. 

Microassays were used in which the final assay volume was 10 pL. Microvolumes were dispensed by using a 100 pL Hamilton syringe and a repeating dispenser. Reactions were carried out in 96-well polystyrene microplates. A typical well contained 2 pL of sodium phosphate buffer, 2 pL of water, and... 

Add 20 fil 20mAf ADP to the sample chamber using a Hamilton syringe. 

Add 20 4mM dinitrophenol to the sample chamber using a Hamilton syringe. At this point the quality of the mitochondrial preparation can be ascertained. Mitochondria in good condition give a substantially higher rate of oxygen consumption when they are uncoupled with dinitrophenol. Explain this observation. 

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