Guinea sensitization test

Di-w-octylphthalate was negative in a skin sensitization test in guinea pigs (Eastman Kodak Company 1978). No further details were provided in this summary report. 

In three cases of acute human exposure from industrial accidents, symptoms included light-headedness, drowsiness, anxiety and apprehension, and nausea slurred speech and vomiting also occurred in one case. In human patch tests, a 23% solution caused severe irritation and possible sensitization. However, guinea pig sensitization tests did not confirm a potential for sensitization. ... 

Applied to the skin of rats and rabbits, the undiluted liquid produced skin irritation, with subsequent degeneration of the epidermis skin sensitization tests in guinea pigs were negative. 

When triethanolamine was applied to the skin of rabbits for 72 hours, there was moderate hyperemia, edema, and necrosis. In a guinea pig sensitization test, there was no evidence of sensitization. In the eyes of rabbits, one drop caused moderate, transient injury at 24 hours. ... 

Life Science Research Submission of Data by CTFA (2-5-50).Dermal Sensitization Test in Guinea Pigs (TEA), 1975... 

Guinea pig tests Indicated that CS had a potential for producing human skin irritation and sensitization.38 39... 

E. I. Dupont de Nemours. 1977b. Primary skin irritation and sensitization testing on guinea pigs. EPA/OTS doc 86-870001118. 

Animals A minimum of 10 animals are used in the treatment group and at least 5 animals in the control group. When fewer than 20 test and 10 control guinea pigs have been used, and it is not possible to conclude that the test substance is a sensitizer, testing in additional animals to give a total of at least 20 test and 10 control animals is strongly recommended. 

Guinea pig sensitization tests require 6-8 weeks and, therefore, take a long time to complete. 

Roberts, D.W., fraginals, R., Lepoittevin, J.-P. and Benezra, C. (1991) Refinement of the relative alkylation index (RAI) model for skin sensitization and application to mouse and guinea-pig test data for alkyl alkanesulphonates. Archives of Dermatological Research, 283, 387—394. 

In the case of contact hypersensitivity, some difficulty arises in extrapolating from the animal sensitization test data to human sensitization risk. This is due to the fact that the published animal test data (primarily guinea pig maximization test results) generally indicate a sensitization potential far greater than the actual human experience would indicate. Lastly, methods for identifying compounds capable of autoimmune responses exist but have not yet been validated. 

S. Kitajima, J. Momma, T. Inoue, Reactivities of the skin-sensitization test in guinea pig (GPMT) as a function of three parameters induction doses (MID), challenge doses (SCD), and direct exposures (DED), Ann. N. Y. Acad. Sci., 919 312-314 (2000). 

Sensitization testing in guinea pigs indicated that blessed thistle is a relatively strong sensitizer. In this testing, crossreactivity to "a considerable number" of other plants in the Asteraceae family was observed (Zeller et al. 1985). Blessed thistle contains the compound cnicin, a sesquiterpene lactone. Such compounds are responsible for allergic contact dermatitis associated with a number of species of the Asteraceae family (Gordon 1999). 

In sensitization tests in guinea pigs, the compounds alan-tolactone and isoalantolactone showed sensitizing activity after intradermal injection, with no sensitization shown... 

Coltsfoot demonstrated a weak sensitizing capacity in a guinea pig sensitization test (Zeller et al. 1985). 

Studies in rabbits show that the ASC germicidal gel product and/or sodium chlorite have the potential to cause skin and eye irritation (Abdel-Rahman et al., 1982b Seta et al., 1991). A sensitization test of the germicidal liquid and gel in guinea-pigs involved intradermal injection of an initial dose of 50 mg followed by nine injections of 100 mg of each test substance over a period of 3 weeks. No sensitization effects were seen. Necrosis was seen following injection of the... 

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