Glycoforms

Comparative Western analysis of samples directly extracted from tissue without intermediate purification [Pg.276]

Figure 3. Dionex HPAEC profiles following 45 minute digestion of 1.25% PGA by 0.1 RGU each of 3 different glycoforms of F. Moniliforme EPGs.

Figure 6. Dionex HPAEC profiles following 48 hour digestion of 1.25% PGA by 0.1 RGU each of 4 different EPGs inhibited by cv cannelino cultured cell PGIP. A and B) F. moniliforme EPG glycoforms, C) deglycosylated S. cerevicae expiressed EPG, D) S. cerevicae expressed EPG.

Yim, K. W., Fractionation of the human recombinant tissue plasminogen activator (rtPA) glycoforms by high-performance capillary zone electrophoresis and capillary isoelectric focusing, /. Chromatogr , 559, 401, 1991. 

Watson, E. and Yao, F., Capillary electrophoretic separation of human recombinant erythropoietin (r-HuEPO) glycoforms, Anal. Biochem., 210, 389, 1993. 

Bateman, K.R, White, R.L., Yaguchi, M., and Thibault, R, Characterization of protein glycoforms by capillary-zone electrophoresis-nanoelectrospray mass spectrometry, /. Chromatogr. A, 794, 327, 1998. 

Although carbohydrates and their corresponding conjugated glycoforms have long been regarded as only space-filling matrices or post-transcriptional accessory... 

Plant-based production systems are now being used commercially for the synthesis of foreign proteins [1-3]. Post-translational modification in plant cells is similar to that carried out by animal cells plant cells are also able to fold multimeric proteins correctly. The sites of glycosylation on plant-produced mammalian proteins are the same as on the native protein however, processing of N-linked glycans in the secretory pathway of plant cells results in a more diverse array of glycoforms than is produced in animal expression systems [4]. Glycoprotein activity is retained in plant-derived mammalian proteins. 

Synagis, a humanized monoclonal antibody, consists of several glycoforms. This indicates that a certain degree of microheterogeneity would be acceptable by the FDA. 

Finally, work that may facilitate understanding the role of oq-acid glycoprotein variants in inter-individual variations in plasma protein binding, pharmacokinetic behavior, and drug action has been described. A capillary zone electrophoresis method that allows for the determination of 11 intact forms (i.e., isoforms, glycoforms) of oq-acid glycoprotein has been described [84],... 

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