Recombinant human erythropoietin glycoforms

Sanz-Nebot V, Benavente F, Vallverdn A, et al. (2003). Separation of recombinant human erythropoietin glycoforms by capillary electrophoresis nsing volatile electrolytes. Assessment of mass spectrometry for the characterization of erythropoietin glycoforms. Analyt. Chem. 75 5220-5229. 

Several pure proteins have been purified and checked for their purity, microheterogeneity, or diagnostic significance by CE. In this case, coated capillaries are preferred for this separation. Many of the proteins can be analyzed by CE however, sensitive detectors such as fluorescence are necessary [68-70]. Examples of proteins studied by CE separately or in a profile are transferrin isoforms, which are important as markers of alcoholism [71], a-1 antitrypsin [72], recombinant human erythropoietin glycoforms that stimulates erythopiosis [73], plasminogen tissue activator [74], prions [75], urothelial carcinoma proteins in urine [76,77], and numerous urinary proteins [78]. 

Madajova, V. et al.. Fractionation of glycoforms of recombinant human erythropoietin by preparative capillary isotachophoresis. Electrophoresis, 26, 2664, 2005. 

D. Macmillan, R. M. Bill, K. A. Sage, D. Fern, and S. Flitsch, Selective in vitro glycosylation of recombinant proteins Semi-synthesis of novel homogeneous glycoforms of human erythropoietin, Chem. Biol., 8 (2001) 133-145. 

USA erythropoietin alfa hematopoiesis Epogen Epoade, Epopen, Eprex, Erypo, Espo, Globuren, Procrit Recombinant human 1-165 EPO using human clone lambdaHEPOFL 13, the glycoform is alpha and expressed in a mammalian cell line Hematopoiesis... 

Watson, E. and Yao, F., Capillary electrophoretic separation of human recombinant erythropoietin (r-HuEPO) glycoforms, Anal. Biochem., 210, 389, 1993. 

---