Transferrin glycoforms

Chromatographic Measurement of Transferrin Glycoforms for Detecting Alcohol Abuse and Congenital Disorders of Glycosylation... 

Figure 6.4 Transferrin glycoform patterns for serum samples from CDG-I patients by HPLC. HPLC traces for (a) a control serum sample from a light drinker (solid line) and from a heavy drinker with elevated asialo- and disialotransferrin and a measurable monosialotransferrin (broken line), (b-d) serum samples from three CDG-Ia patients showing elevated asialo- and disialotransferrin and reduced tetrasialotransferrin, (e) a serum sample from a CDG-Ib patient showing elevated asialo- and disialotransferrin (solid line) and another sample from the same patient after mannose therapy (broken line) and (f) a serum sample from a CDG-Ig patient showing elevated asialo- and disialotransferrin. Parts (a), (d), (e) and (f) reproduced from A. Helander, J. Bergstrom and H.H. Freeze, Clin. Chem., 50, 954-958 (2004), by permission of the American Association of Clinical Chemistry...

Improved HPLC methods for the separation and quantihcation of transferrin glycoforms, based on anion-exchange chromatographic separation followed by selective photometric detection at 460 70 nm or MS detection, are useful for the detection and monitoring of... 

A. Helander, J. Bergstrom and H.H. Freeze, Testing for congenital disorders of glycosylation by HPLC measurement of serum transferrin glycoforms, Clin. Chem., 50, 954-958 (2004). 

Sanz-Nebot, V. et al.. Characterization of human transferrin glycoforms by capillary electrophoresis and electrospray ionization mass spectrometry, J. Chromatogr. B, 798, 1, 2003. 

---