Glycans lectins

Ramirez-Soto, D. and Poretz, R.D. (1991) The (l-3)-linked (X-L-fucosyl group of the IV-glycans of the Wistaria floribunda lectins is recognised by a rabbit antiserum. Carbohydate Research 213, 27-36. 

J. J. Calvete, C. F. Santos, K. Mann, T. B. Grangeiro, M. Nimtnz, C. Urbanke, and B. S. Cavada, Amino acid sequence, glycan structure, and proteolytic processing of the lectin of Vatairea macrocarpa seeds, FEBS Lett., 452 (1998) 286-292. 

Retention of native carbohydrate structure also is important in applications that utilize the conjugated polysaccharide in binding studies with receptors or lectins. In these cases, the carbohydrate should be modified at limited sites, preferentially only at its reducing end. Section 4.6 of this chapter discusses glycan conjugation techniques in greater detail. 

Glycan array allows for the definition of fine differences in specificity among related lectins giving clues about the basis for differences in their biological activities. For example comparison of a set of human galectins demonstrated that Gal-1, Gal-2, and Gal-3 all bind /V-acetyllactosamine but... 

The high sensitivity and reproducibility of currently used instruments also allows to directly monitor the binding of small ligands, i.e. disaccharides or monosaccharides, to immobilized lectins.75 77 Fig. 3 illustrates the binding of small glycans to BclA lectin from Burkholderia cenocepacia.26... 

Analytical tools have been developed in order to identify carbohydrate structures as well as carbohydrate-binding proteins and to understand their underlying structure-function relationships of protein-carbohydrate and carbohydrate-carbohydrate interactions lectin arrays [16], glycan microarrays [17, 18], glyco-nanoparticles [19], frontal affinity chromatography [20] and carbohydrate tools for metabolic labeling [21]. 

Teraguchi, S., Shin, K., Fukuwatari, Y., and Shimamura, S. (1996). Glycans of bovine lacto-ferrin function as receptors for the type 1 fimbrial lectin of Escherichia coli. Infect. Immun. 64,1075-1077. 

It is possible that membrane lectins play a role in the binding of glycans during these processes. Protein fractions having lectin activity have been... 

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