DNA-binding studies

For a review, see M. Casely-Hayford, M. Searcey, The azinomycins. Discovery, synthesis and DNA-binding studies. in DNA and RNA hinders. From small molecules to drugs, Wiley-VCH, Weinheim, 2002. pp. 676 696. 

Liu, H.-Q., Cheung, T.-C., Peng, S.-M. and Che, C.-M. (1995) Novel luminescent cyclometallated and terpyridine gold(III) complexes and DNA binding studies. Journal of the Chemical Society, Chemical Communications, 1787-1788. 

DNA-binding studies on the series of isomeric [Ru(rj6-arene) Cl(en)]+ compounds with o-, m-, or p-terphenyl as the arene further illustrate the importance of the hydrophobicity and... 

K. J. Breslauer, D. P. Remeta, W. Y Chou, R. Ferante, J Curry, D. Zauczkowski, J. G. Snyder, and L. A. Marky, Enthalpy-entropy compensations in drug-DNA binding studies, Proc. Natl. Acad. Aci. USA 84 892 (1987). 

Methyl iodide is considered a potent methylating agent it methylates hemoglobin in experimental animals and humans. In DNA binding studies in rats, adducts were found in all organs examined, with the highest levels in the stomach and forestomach, after both oral and inhalation administration. It is mutagenic... 

Subsequently, DFT methods (B3LYP functional ) were employed to compute (1) natural charges from which changes in charges are mapped out for comparison with the NMR-based conclusions, (2) GIAO-NMR to predict the chemical shifts for comparison with the experimental results, and (3) nuclear-independent chemical shift (NICS) in order to evaluate relative aromaticity in different rings. Finally, solvent effects were estimated by the polarized continuum model (PCM). In selected cases, parallel DNA-binding studies (with MCF-7 human mammary... 

Taken together, these studies represent the ground work for building up a carbocation-based structure/reactivity database. Additional comparison of these trends with the data obtained by comparative DNA-binding studies and by tumor-igenicity and mutagenicity assays will be needed in an effort to draw a parallel. 

The preparation of an imidazole-pyrrole dipeptide segment for DNA binding studies is shown in Scheme 3[10 and described in Section 11.2.1. 

The fact that GBF-1 bound to the cab-E G-box-like sequence, but bound only weakly to the USF recognition sequence and the G-box-like elements of the A. majus chalcone synthase promoter, contrasted with results reported for the plant nuclear factor CG-1 (Staiger et al., 1989). This latter factor was shown to interact with both the USF recognition sequence and one of the G-box-like elements of the A. majus chalcone synthase promoter it did not interact with the cab-E motif. These cumulative DNA-binding studies indicate that GBF-1 and CG-1 have different DNA-binding properties. 

Table I. Summary of Results of DMBA-DNA Binding Studies in Various Cellular and Subcellular Systems...

We also used DNA binding studies to show that tn vitro sub-cellular activation systems do not qualitatively duplicate target tissue activation and thus may not be adequate as activation systems for short-term tests for detection of chemical carcinogens. 

The most extensively studied hormone reponsive elements are found = 200 bp from the transcription start site in the long terminal repeat (LTR) of the mouse mammary tumor virus (MMTV). They are responsive to GR as shown by in vitro DNA binding studies with purified GR [131-133] and by in vivo response data with deletion mutants [134—139], From these studies three important functional domains in the LTR of MMTV have been identified two binding sites for GR - a promoter distal binding site and a promoter proximal binding site - and a third site which binds nuclear factor-one (NF-1). 

When I was still at Columbia I was working on chiral metal complexes bound to DNA and I was also doing some studies in collaboration with Nick Turro, my wonderful colleague at Columbia, on the photophysical properties of ruthenium complexes bound to DNA. The complexes I was using in DNA binding studies could be considered as derivatives of those that had been used by Henry Taube in his classic studies of electron transfer... 

All of the in vitro DNA binding studies to date have shown that small Maf binds to DNA more readily as a heterodimer with either p45 NF-E2, Nrfl, Nrf2,... 

Broomhead, J. A., Rendina, L. M., Webster, L. K. Dinuclear complexes of platinum having anticancer properties. DNA-binding studies and biological activity of bis(4,4 -dipyrazolylmethane-7V,7V )-bis[dichloroplatinum(ll)] and related complexes. J. Inorg. Biochem. 1993,49,221-234. 

DNA-binding Studies. Another important subject in the field of residue... 

Table II DNA-binding studies on furazolidone Pig hepatocytes were incubated with 50 nM C-furazolidone for 16 hr (starting 26 hr after isolation), or C-leucine for 40 hr (starting 4 hr after isolation). Cells were homogenized, the chromatin collected by centrifugation and DNA purified by phenolic extraction...

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