Glycan Structures

Glycans are synthesized in a template independent manner by the concerted action of glycosynthetic and glycolytic enzymes, thus there is no mechanism, at present, to amplify glycan structures that allows facilitated structure-function studies. 

CCSD http //www.boc.chem.uu.nl/sugabase/caibbank.html 

Glycan DB http //www.functionalglycomics.oig/glycomics/mole cule/jsp/carbohydiate/carbMoleculeHome.jsp GlycoBase http //uslt.univ-lille 1. fr/glycobase/ 

Genertd structure information Prim structures, analysis Glycomic DB, modeling and tools Carbohydrate structures from PDB Glycan structures, search for substructures 

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Matsuda, K., Takahashi, N., Tsukamoto, Y., Honma, H., and Kohri, K., N-glycan structures of osteopontin from human bone, Biochem. Biophys. Res. Commun., 268, 814, 2000. 

Yuen, C.T., Stoning, P.L., Tiplady, R.J. et al. (2003) Relationships between the A-glycan structures and biological activities of recombinant human erythropoietins produced using different culture conditions and purification procedures. British Journal of Haematology, 121 (3), 511-526. 

T. A. Gerken, Kinetic modeling confirms the biosynthesis of mucin core 1 (/i-Gal (1 -3)x-Gal YAc-O-Ser/Thr) O-glycan structures are modulated by neighboring glycosylation effects, Biochemistry, 43 (2004) 4137—4142. 

J. J. Calvete, C. F. Santos, K. Mann, T. B. Grangeiro, M. Nimtnz, C. Urbanke, and B. S. Cavada, Amino acid sequence, glycan structure, and proteolytic processing of the lectin of Vatairea macrocarpa seeds, FEBS Lett., 452 (1998) 286-292. 

Figure 10.3 Whole-mass analysis of a monoclonal antibody. (A) Direct infusion of the antibody generates an envelope of high m/z ions ranging from 2000 to 3500. Deconvolution of the ion current signal gives the mass of the complete native molecule (147, 100.97 Da) and resolves some heterogeneity linked to the A-glycan structures. The major forms are consistent with molecules carrying biantennary structures capped with 0, 1, or 2 hexose (G = galactose) residues. (Data generated on an ESI-Q-Star instrument, Sciex-Applied Biosystems.)...

Figure 10.6 Peptide mapping of PIXY321. (A) Cleavage of the primary sequence with Lys-C endoprotease results in 14 theoretical fragments. (B) As detailed in the text, LC-MS analysis of the Lys-C digest shows that a few peptides, notably LI, L7, and L14, elute at several retention times due to heterogeneity of both their glycan structures and amino acid sequence. The insets illustrate the complexity of the mass spectra of glycopeptide LI eluting under peaks 17 (inset C) and 18 (inset D).

Figures Selective protein modification using a keto amino acid, p-acetyl-L-phenylalanine. (a) Labeling of fluorescein hydrazide to the Z domain protein. Only the mutant protein containing p-acetyl-L-phenylalanine was labeled and became fluorescent, (b) A general method for preparing glycoprotein mimetics with defined glycan structure.

In order to mimic multiantennary oligosaccharide topology with greater variety of glycan structures and geometry, Roy et al. [77] used solid phase peptide syn-... 

Scheme 12 Important Mucin Glycoprotein Glycan Structures...

Misaki IL, Nagaya H., Fujiyama K., Yanagihara I., Honda T. and Seki T. (2003) N-Hnked glycan structures of mouse interferon-p produced by Bombyx mori larvae. Biochem. Biophys. Res. Commun. 311,979-986. 

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