Glutamate immunocytochemistry

Rousselot P, Poulain DA, Theodosis DT (1994) Ultrastructural visualization and neurochemical characterization of spinal projections of primary sensory afferents from the nipple combined use of transganglionic transport of HRP-WGA and glutamate immunocytochemistry. J Histochem Cytochem 42 115-123. 

Chaudhry F. A., Lehre K. P., van Lookeren Campagne M., Ottersen O. P., Danbolt N. C., and Storm-Mathisen J. (1995). Glutamate transporters in glial plasma membranes highly differentiated localizations revealed by quantitative ultrastructural immunocytochemistry. Neuron... 

Wagner, R., and Deleo, J. A. (1996). Pre-emptive dynorphin and N- me thyl-D- aspartate glutamate receptor antagonism alters spinal immunocytochemistry but not allodynia following complete... 

Gundersen V, Ottersen OP, Storm-Mathisen J (1996) Selective excitatory amino acid uptake in glutamatergic nerve terminals and in glia in the rat striatum quantitative electron microscopic immunocytochemistry of exogenous (D)-aspartate and endogenous glutamate and GABA. Eur J Neurosci 8 758-765. 

Ottersen OP, Laake JH, Storm-Mathisen J (1990) Demonstration of a releasable pool of glutamate in cerebellar mossy and parallel fibre terminals by means of light and electron microscopic immunocytochemistry. Arch Ital Biol /2S 111-125. 

Storm-Mathisen J, Leknes AK, Bore AT, Vaaland JL, Edminson P, Haug F-MS, Ottersen OP (1983) First visualization of glutamate and GABA in neurons by immunocytochemistry. Nature 30/ 517-520. 

Waerhaug O, Ottersen OP (1993) Demonstration of glutamate-like immunoreactivity at rat neuromuscular junctions by quantitative electron microscopic immunocytochemistry. Anat Emhryol (Berl) 7S7i 501-513. 

Petralia RS, Wenthold RJ (1998) Glutamate receptor antibodies. Production and immunocytochemistry. In Ariano MA (Ed), Receptor Localization Laboratory Methods and Procedures. New York Wiley-Liss, pp 46-74. 

Schmitt A, Kugler P (1999) Cellular and regional expression of glutamate dehydrogenase in the rat nervous system non-radioactive in situ hybridization and comparative immunocytochemistry. Neuroscience 92 293-308. 

Schmitt A, Asan E, Puschel B, Kugler P (1997) Cellular and regional distribution of the glutamate transporter GLAST in the CNS of rats nonradioactive in situ hybridization and comparative immunocytochemistry. J Neurosci 77 1-10. 

Ottersen OP, Zhang N, Walberg F (1992) Metabolic compartmentation of glutamate and glutamine morphological evidence obtained by quantitative immunocytochemistry in rat cerebellum. Neuroscience 46 519-534. 

Fredette BJ, Adams FJ, Mugnaini E (1992) GABAergic neurons in the mammalian inferior olive and ventral medulla detected by glutamate decarboxylase immunocytochemistry. J. Comp. Neurol, 321, 501-514. 

Bolam JP, Powell JF, Wu JY, Smith AD (1985) Glutamate decarboxylase-immunoreactive structures in the rat neostriatum a correlated light and electron microscopic study including a combination of Golgi impregnation with immunocytochemistry. J. Comp. Neurol, 237, 1-20. 

Blakely et al. 1987 Ffrench-Mullen et al. 1985). NAG has been observed in mitral cells using immunocytochemistry (Blakely et al. 1987). However, a recent neurophysiological study cast doubt on a transmitter role for NAG (Whittemore and Koerner, 1989) in mitral cells (see below, 2.5.4. Transmitter(s) mediating MOB to PC monosynaptic excitation). A few, unusually small, mitral cells appear to contain aspartate and project to the piriform cortex (Fuller and Price, 1988). Many mitral cells, as well as tufted cells in the EPL, have been reported to express glutamate immunoreactivity (Liu et al. 1989). 

The diversity of neuropeptide transmitters is fairly large, as compared to other, classical neurotransmitters, such as acetylcholine and glutamate. Currently, immunocytochemistry and in situ hybridization are the methods of choice to map neuropeptide expression patterns in the nervous system. A disadvantage of these methods, however, is that they give limited clues to the structural identities of processed and biologically active peptides contained in the cells. Moreover, these techniques do not reveal previously undescribed cotransmitters. 

Wu J.-Y (1983) Preparation of glutamic acid decarboxylase as immunogen for immunocytochemistry, m Neurommunocyto-chemistry (IBRO Handbook Senes Methods in the Neurosciences) (Cuello A. C., ed.), pp. 159-191. John Wiley Sons, Ltd., Sussex. 

Wenthold R. J. and Altschuler R. A. (1983) Immunocytochemistry of Aspartate Aminotransferase and Glutaminase, in Glutamine, Glutamate, and GABA in the Central Nervous System (Hertz L, Kvamme E, McGeer E., and Schousboe A., eds ), pp. 33-50, Alan Liss, New York... 

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