Electron microscopic immunocytochemistry

Keywords Immunohistochemistry Antibody labeling Fluorescence microscopy Fluorescent immunocytochemistry Fluorescent immunohistochemistry Indirect immunocytochemistry Immunostaining 

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Experience has shown that the desired routine sizes are 5, 10, and 15 nm. All these are useful for transmission electron microscope studies and the 5 nm gold is recommended for light microscopy. Gold spheres of 30 nm are occasionally used for scanning electron microscope immunocytochemistry. A1 nm probe is available and might be useful for both light and electron microscopy after silver enhancement. 

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