Glucose yeast cultivation

In the laboratory, the cultivation is conducted in a liquid Hestrin-Schramm medium (glucose, yeast extract, peptone, citric acid and sodium phosphate) at pH 5 and 28°C. The cultures are grown in static containers or air flow (airlift bioreactors). The growing time depends on the desired thickness of the ensuing cellulose membrane. Several studies can be found on the growth kinetics of those microorganisms, based on fermentative processes [5]. 

For small-scale cultivation of S. insignis, our laboratory has observed moderate anrhracycline production in GYE (glucose-yeast extract) medium consisting of 6.25% cerelose, 1.25% yeast extract, 0.33% NaO, and 0.625% GaCOj, pH 7.2 (78). Additional media useful for cultivation and fermentation of S. imignis also have been described pre-viousty (77,78). 

Pseudomonas spp. DSM 6611 and 6978 and Rhodococcus ruber DSM 44541 were cultivated in shaking flasks for 3 days at 30 °C with shaking at 120 rpm in YPG medium containing 10 g yeast extract, 10 g bacteriological peptone, 10 g glucose. 

Production of Lignin Peroxidase. Medium for the inoculum was rich in yeast extract (25 g/1) and glucose (25 g/l) to promote maximal growth of the mycelia. The inoculum of Phanerochaete chrysosporium ATCC 24725 was first cultivated for 3 days at 30°C in five litres of medium divided in five shake flasks. The shake flask batches were transferred to a 100 litre bioreactor and cultivated again for 3 days at 30°C. The batches were stirred and aerated to obtain maximal growth of mycelia. 

As for the cultivation of other types of marine microorganisms, e.g., those with a specific potential for the production of biologically active metabolites, predominantly small-scale experiments (shake flasks) have been described. Alternatively, artificial seawater or 25 50 75 90% natural seawater has served as a basis for nutrient media. The concentrations of carbon and nitrogen sources reached up to 2 % (w/w) starch, glucose, molasses, glycerol, soybean oil, yeast extract, malt extract, beef extract, peptone, cornsteep liquor and NZ-amine. In the absence of artificial or natural seawater, high concentrations of... 

Fermentation procedures for preparing uridine 5 -(2-acetamido-2-deoxy-a-D-glucopyranosyl pyrophosphate) have also been reported. One of them255 involves the cultivation of Helminthosporium sativum in the presence of 2-amino-2-deoxy-D-glucose and the antibiotic polyoxin the latter is an inhibitor of chitin biosynthesis. The other256 utilized the incubation of yeast cells with uridine 5 -phosphate in the presence of an excess of 2-amino-2-deoxy-D-glucose and inorganic phosphate.257... 

You are going to cultivate yeast, Saccharomyces cerevisiae, by using a 10 m -fermenter your company already owns. You want to find out the amount of ethanol the fermenter can produce. Therefore, a chemostat study was carried out and the Monod kinetic parameters for the microorganism grown in the glucose medium at 30°C, pH 4.8, were found to be Ks = 0.0025 g/L and /imax = 0.25 h-1. The ethanol yield (YP/S) is 0.44 (g/g) and cell yield (Yx/S) is 0.019 (g/g). The inlet substrate concentration is 50 g/L-... 

These examples indicate the in situ applicability of enzyme electrodes however, numerous problems have still to be solved. At present, coupling of enzyme sensors for fermentation control in a bypass arrangement appears to be more favorable [412]. Following this concept, an invertase thermistor incorporating a sterilizable filter unit has been developed [413] for the monitoring of alcoholic fermentation by immobilized yeast cells. Another thermistor has been successfully used for on-line glucose measurement under real cultivation conditions of Cephalosporium acremonium [414]. Similar calorimetric devices are suitable for other fermentation processes and in environmental analysis. 

The general ability to accumulate Upids varies between yeast species and is dependent on the substrate, nutrient hmitation, and the environmental conditions, such as pH and temperature [62]. While Lipomyces starkeyi, Rho-dosporidium toruloides, and Trichosporon cutaneum grown on glucose reach total lipid contents between 40% and 65% [68-70], Y. lipolytica accumulates only up to 20% of lipids [71]. On the other hand, lipid content of Y. lipolytica cultivated on glycerol increases to 40% [72]. For further readings, we recommend the reviews by Papanikolaou and Aggelis [73,74]. 

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