Schramm-Hestrin medium

Fig. 8 Schematic comparison of cellulose structure formation of BC synthesized in a Hestrin-Schramm (HS) medium (Fig. 3), b glucomannan-modified HS medium. Reprinted with permission from [44]...

The never-dried NOC template was put into Schramm-Hestrin (SH) medium (Hestrin and Schramm 1954) at pH 6.0, and the solvent was exchanged and finally maintained in a Petri dish until used for the study. 

In 1886, A. J. Brown [11] discovered BC as a biosynthetic product of Glu-conacetobacter xylinus strains. He identified a gelatinous mass, formed on the solution during the vinegar fermentation as cellulose. In the middle of the 20th century, Hestrin and Schramm developed a special culture medium for... 

Aceiobacter xylinus DSM 14666 static culture. 28°C, 14 days T Hestrin-Schramm-medium... 

In the laboratory, the cultivation is conducted in a liquid Hestrin-Schramm medium (glucose, yeast extract, peptone, citric acid and sodium phosphate) at pH 5 and 28°C. The cultures are grown in static containers or air flow (airlift bioreactors). The growing time depends on the desired thickness of the ensuing cellulose membrane. Several studies can be found on the growth kinetics of those microorganisms, based on fermentative processes [5]. 

The Hertrin-Schramm medium is a standard medium used for Acetobactor cultivation and formation of BC (Hestrin and Schramm 1954, Shirai et al. 1997). Glucose or sucrose is usually employed... 

Figure 10 (a) Cellulose pellicle of thickness 10 mm produced by AX at the top of the Hestrin-Schramm (HS) culture medium, (b) Film specimen of 2- to 5-mm thickness obtained by cutting a part of the pellicle at the air side and immersed in D2O after replacing H2O with D2O. The prokaryotic Xs shown in part (c) exist in the HS medium or in the pellicle close to the interface between the medium and the pellicle in part (a). 

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