Glucose transporter characterization

Fukumoto, H., et al. Cloning and characterization of the major insulin-responsive glucose transporter expressed in human skeletal muscle and other insulin-responsive tissues. J. Biol. Chem. 1989, 264, 7776-7779. 

Doege, H., et al. Characterization of human glucose transporter (GLUT) 11 (encoded by SLC2A11), a novel sugar-transport facilitator specifically expressed in heart and skeletal muscle. Biochem. J. 2001, 359, 443-449. 

Wu, X., et al. Cloning and characterization of glucose transporter 11, a novel sugar transporter that is alternatively spliced in various tissues. Mol. Genet. Metab. 2002, 76, 37—45. 

INTESTINE Characterization of a membrane potassium ion conductance in intestinal secretory cells using whole cell patch-clamp and calcium-sensitive dye techniques, 192, 309 isolation of intestinal epithelial cells and evaluation of transport functions, 192, 324 isolation of enterocyte membranes, 192, 341 established intestinal cell lines as model systems for electrolyte transport studies, 192, 354 sodium chloride transport pathways in intestinal membrane vesicles, 192, 389 advantages and limitations of vesicles for the characterization and the kinetic analysis of transport systems, 192, 409 isolation and reconstitution of the sodium-de-pendent glucose transporter, 192, 438 calcium transport by intestinal epithelial cell basolateral membrane, 192, 448 electrical measurements in large intestine (including cecum, colon, rectum), 192, 459... 

Skelly, P.J., Kim, J.W., Cunningham, J. and Shoemaker, C.B. (1994) Cloning, characterization, and functional expression of cDNAs encoding glucose transporter proteins from the human parasite Schistosoma man-soni. Journal of Biological Chemistry 269, 4247-4253. 

Mantych, G.J., G.S. Hageman, and S.U. Devaskar. 1993. Characterization of glucose transporter isoforms in the adult and developing human eye. Endocrinology 133 600. 

Gould GW, Thomas HM, Jess TJ, Bell GI (1991), Expression of human glucose transporters in Xenopus oocytes. Kinetic characterization and substrate specificities of the erythrocyte, liver, and brain isoforms, Biochemistry 30 5139-5145. 

Parker, C., Barnell, W. O., Snoep, J. L., Ingram, L. O., and Conway, T. 1995. Characterization of the Zymomonas mobilis glucose facilitator gene product (gif) in recombinant Escherichia colt examination of the transport mechanism, kinetics and the role of glucokinase in glucose transport. Mol. Microbiol., 15, 759-802. 

Bowsher CG, Scrase-Field EF, Esposito S, Ernes MJ, Tetlow IJ. Characterization of ADP-glucose transport across the cereal endosperm amyloplast envelope. J. Exp. Bot. 2007 58 1321-1332. Hylton C, Smith AM. The rb mutation of peas causes structural and regulatory changes in ADP-Glc pyrophosphorylase from developing embryos. Plant Physiol. 1992 99 1626-1634. 

Inukai K, Katagiri H, Takata K, Asano T, Anai M, et al. 1995. Characterization of rat GLUTS and functional analysis of chimeric proteins of GLUTl glucose transporter and GLUTS fructose transporter. Endocrinology 136 4850-4857. 

Another form of facilitated diffusion involves membrane proteins called carriers (sometimes referred to as passive transporters). In carrier-mediated transport, a specific solute binds to the carrier on one side of a membrane and causes a conformational change in the carrier. The solute is then translocated across the membrane and released. The red blood cell glucose transporter is the best-characterized example of passive transporters. It allows D-glucose to diffuse across the red blood cell membrane for use in glycolysis and the pentose phosphate pathway. Facilitated diffusion increases the rate at which certain solutes move down their concentration gradients. This process cannot cause a net increase in solute concentration on one side of the membrane. 

Langford, C. K., Ewbank, S. A., Hanson, S. S., Ullman, B. and Landfear, S. M. (1992) Molecular characterization of two genes encoding members of the glucose transporter superfamily in the parasitic protozoan Leishmania donovani. Mol. Biochem. Parasitol. 55 51-64. 

The existence of an Na gradient is essential for Na -coupled glucose transport in other systems. This gradient is usually maintained by an Na" /K -ATPase that pumps more Na" ions out of the cell than ions in. However, Na" and glucose transport across the A. suum intestine is not affected by high levels of ouabain (152), indicating that, if an Na" /K -ATPase regulates the concentrations of these ions, the enzyme is distinct from that characterized in vertebrates and most other invertebrates. 

Bachem, S. Faires, N. Stulke, J. Characterization of the presumptive phosphorylation sites of the Bacillus subtilis glucose permease by site-directed mutagenesis implication in glucose transport and catabolite repression. FEMS Microbiol. Lett, 156, 233-238 (1997)... 

Fryer, L.G. Foufelle, R Barnes, K. Baldwin, S.A. Woods, A. Carling, D. Characterization of the role of the AMP-activated protein kinase in the stimulation of glucose transport in skeletal muscle cells. Biochem. J., 363, 167-174 (2002)... 

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