Glucose oxidase carbon paste modified electrode

Figure 4. (left) Steady state response to 31.5 mM glucose of the ferrocene-modified poly(siloxane) / glucose oxidase / carbon paste electrodes at several applied potentials. The relay systems are indicated next to each curve, which is the mean result for four electrodes. 

Figure 8. (right) Glucose calibration curves for the ferrocene-modified poly(ethylene oxide)/glucose oxidase/carbon paste electrodes at E =... 

Karan et al. [10] reported glucose sensors using quinone modified poly-siloxane (Fig. 3.8a-A) and acrylonitrile-ethylene (Fig. 3.8a-B) co-polymers and glucose oxidase. Sensors constructed with glucose oxidase and quinone modified polysiloxane were considerably more efficient than those using acrylonitrile-ethylene system to transfer electrons from reduced glucose oxidase to a conventional carbon paste electrode. Their results coincide with those described previously for the ferrocene-modified polysiloxane system. The excellent flexibility of poly(siloxane) allows it to function as an efficient... 

In this article, we describe the preparation and use of amperometric electrodes for sensing the analytically significant substrate glucose based on a PEG-modified glucose oxidase (GOD) (4,5) and on a lipid-modified GOD (6). The PEG-modified GOD has been incorporated into a carbon paste (CP) electrode the modified enzyme exhibits higher activity in a hydrophobic CP matrix than hydrophilic, native GOD (7-10). The lipid-modified GOD has been immobilized on a glassy carbon (GC) electrode with a thin Nafion overcoat. The water-insoluble modified enzyme is far more stable between the electrode surface and the polymer layer than native GOD (77). 

S.Q. Liu and H.X. Ju, Reagentless glucose biosensor based on direct electron transfer of glucose oxidase immobilized on colloidal gold modified carbon paste electrode. Biosens. Bioelectron. 19, 177-183 (2003). 

Saby C, Mizutani F, Yabuki S. Glucose sensor based on carbon paste electrode incorporating poly(ethylene glycolj-modified glucose oxidase and various mediators. Analytica Chimica Acta 1995 304 33-39. 

Mizutani, R, S. Yabuki, and S. lijima (1995). Amperometric glugose-sensing electrode based on carbon paste containing poly(ethylene glycol)-modified glucose oxidase and cobalt octaethoxyphthalocyanine. Anal. Chim. Acta 300, 59-64. 

CEEC has been used extensively for the detection of small molecules in blood and urine. Pulsed amperometric detection at a gold electrode was used to detect glucose in blood following capillary electrophoresis separation [59]. It was also detected using a glucose oxidase-modified carbon paste electrode [33]. As previ-... 

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