Glucose oxidase electrodes

W. Sung and Y. Bae, A glucose oxidase electrode based on electropolymerized conducting polymer with polyanion-enzyme conjugated dopant. Anal. Chem. 72, 2177-2181 (2000). 

FIGURE 5.25. Avidin-biotin construction of a monolayer glucose oxidase electrode with an attached ferrocenium cosubstrate and cyclic voltammetric response in a phosphate buffer (pH 8) at 25°C and a scan rate of 0.04 V/s. a attached ferrocene alone, h In the presence of 0.5 M glucose, c Variation of the inverse of the plateau current with the inverse of substrate concentration. Adapted from Figure 1 in reference 24, with permission from the American Chemical Society. 

Figure 4. Calibration curve for AQ-glucose oxidase electrode, prepared by casting 25 il of 1% AQ solution and 12 U of enzyme. The film was dried at 50°C during 30 min in an oven. Inset Linear portion of the calibration curve.

Electrochemical Characterization of Ferrocene Derivatives in a Perfluoropolymer Glucose Oxidase Electrode... 

Figure 3-7. Assembly of an electrically-contacted polyaniUne-reconstituted glucose oxidase electrode. Reproduced with permission from ref. 34. Copyright 2002 American Chemical Society.

Figure 3-8. Cyclic voltammograms corresponding to the bioelectrocatalyzed oxidation of variable concentrations of glucose by the integrated, electrically-contacted polyaniline-reconstituted glucose oxidase electrode. Glucose concentrations correspond to (a) 0 mM, (b) 5 rnM. (c) 10 mM, (d) 20 mM, (e) 35 inM. (1) 50 mM. Reproduced with permission from ref. 34. Copyright 2002 American Chemical Society.

Figure 3-10. The assembly of an Au-nanoparticle (1.4 nm) electrically contacted glucose oxidase electrode by (a) the primary reconstitution of apo-GOx on the FAD-functionalized Au-nanoparticle, and the immobilization of the enzyme-nanoparticle hybrid on an electrode surface (b) the primary immobilization of the FAD-modified Au-nanoparticle on tire electrode surface and the surface reconstitution of apo-GOx on the functionalized electrode. Reproduced with permission from ref. 41. Copyright 2003, AAAS.

Figure 3-30. Organization of a photoswitchable glucose oxidase electrode for the bioelectrocatalyzed oxidation of glucose (A) The synthesis of the photoisomerizable nitrospiropyran-FAD cofactor. (B) The reconstitution of apo-glucose oxidase, apo-GOx, with the photoisomerizable FAD-cofactor (20a). (C) The assembly of the reconstituted photoisomerizable GOx on an electrode surface and the photoswitching of the bioelectrocatalytic function of the enzyme electrode in the presence of ferrocene carboxylic acid (21) as mediator.

Figure 3-32. (A) The assembly of an electro switchable, electrically-contacted, glucose oxidase electrode by the reconstitution of apo-glucose oxidase, apo-GOx, on PQQ-FAD units linked to a polyacrylic acid fihn that includes Cu -ion attached to the fihn. The conductivity of the film and the electrical contacting of the enzyme is accomplished by applying a potential of -0.5 V vs. SCE and the generation of Cu-clusters in the film. Reproduced with permission from ref. 89. Copyright 2003 American Chemical Society.

A glucose sensor system consisting of an oxygen electrode and a glucose oxidase electrode was recently implanted into the vena cava of a dog (McKean and Gough, 1988). The sensors operated in the potentio-static mode and were connected to an implantable telemetry system. Both the enzyme stability and the power consumption allowed operation of the system for three months. 

F. Mizutani and M. Michihiko, Simultaneous determination of glucose and lactose in sour milk using an immobihzed glucose oxidase electrode combined with P-galactosidase-attached measuring cell, Bunseki Kakagu, 39 (11), 729-34 (1990). 

Figure 14-8. Response of a graphite-glucose oxidase electrode in an automatic flow-injection system.

Romette, J. L., Froment, B., Thomas, D., Glucose Oxidase Electrode. Measurements of Glucose in Samples Exhibiting High Variability in Oxygen Content , Clin. Chim. Acta 95 (1979) 249-253. 

Wollenberger, U., Scheller, E, Pfeiffer, D., Bogdanovskaya, V. A., Tarasevich, M. R., Hanke, G., Laccase/Glucose Oxidase Electrode for Determination of Glucose , Anal. Chim. Acta 187 (1986) 39-45. 

J.Rishpon and S.Gottesfeld, Investigation of polypyrrole/glucose oxidase electrodes by ellipsometiic,... 

S.Mu, H.Xue and B.Qian, bioelectrochemical responses of the polyaniline glucose oxidase electrode, J. 

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