6-Phospho-D-gluconate

The 2-keto-3-deoxy-aldonic acid (phosphate) aldolases from Pseudomonas strains - 3-deoxy-2-keto-L-arabonate (F.C 4.1.2.18), 3-deoxy-2-keto-D-xylonate (EC 4.1.2.28), 3-deoxy-2-keto-6-phospho-D-gluconate (EC 4.1.2.14) and 3-deoxy-2-keto-6-phospho-D-galactonate aldolase (EC 4.1.2.21) - appear to be specific even for the acceptor components, but allow stereoselective syntheses of the respective natural substrates29. 

Lactate 6-phospho-D-gluconate Lactate dehydrogenase ferri- 81)... 

This enzyme [EC 1.1.1.43] catalyzes the reaction of 6-phospho-D-gluconate with NAD(P)+ to produce 6-phos-pho-2-dehydro-D-gluconate and NAD(P)H. 

EC 1.1.1.44 Phosphoglucuronate dehydrogenase 6-Phospho-D-gluconate+NADP+ D-ribose-5-phosphate + C02 + N ADPH Mammary gland SM... 

Keto-3-deoxy-6-phospho-D-gluconate Aldolase= 2-Keto-4-bydroxyglutarate Aldolase... 

The 2-keto-3-deoxy-6-phospho-D-gluconate (11) aldolase (KDPGlc aldolase or KdgA EC 4.1.2.14) provides the basis of the Entner-Doudoroff pathway that is used by many species of bacteria for the degradation of 6-phosphogluconate... 

The results obtained with the biosensor for five different honey samples were compared with those obtained with a commercial enzyme kit from R-biopharm using spectrophotometric detection (Table 16.1). This method is based on the phosphorylation of gluconate by gluconate kinase in the presence of ATP, then the 6-phospho-D-gluconate produced was oxidized with NADP+ by 6-phosphogluconate dehydrogenase. The reduced form of NADP, NADPH, formed in this oxidation reaction is measured spectrophotometrically at 340 nm. 

The unit activity is defined as the amount of enzyme which oxidizes 1 /rmole of glucose-6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP+ at pH 7.8 and 30 C (specific activity up to about 400 U/mg). If NAD+ is used as coenzyme, activity increases by a factor of 1.8. 

The analogous reduction experiment carried out with pyruvate plus 2-keto-3-deoxy-6-phospho-D-gluconate aldolase results in almost equal reduction at both diastereotopic faces of the ketimine (178). Apparently, solvent accessibility to the ketimine is not uniformly restricted among all aldolases. 

Phosphogluconate Dehydrogenase An enzyme of the oxidoreductase class that catalyzes the reaction 6-phospho-D-gluconate and NADP+ to yield D-ribulose 5-phosphate, carbon dioxide, and NADPH. The reaction is a step in the pentose phosphate pathway of glucose metabohsm. (From Borland, 27th ed) EC 1.1.1.43. [nih]... 

Adachi O, Osada K, Matsushita K, Shinagawa E, Ameyama M (1982) Purification, crystallization and properties of 6-phospho- D-gluconate dehydrogenase from Gluconobacter suboxydans. Agric Biol Chem 46 391-398... 

Phospho-D-gluconic acid is formed from gluconic add by yeast glucono-kinase and from glucose 6-phosphate by the corresponding dehydrogenase or by bromine oxidation ... 

Stereocomplementary sets of pyravate dependent aldolases. /V-acetyIneuraminic acid aldolase (NeuA EC 4.1.3.3), 2-keto-3-deoxy-manno-octosonate (KDO) aldolase (KdoA EC 4.1.2.23), 2-keto-3-deoxy-6-phospho-D-gluconate aldolases (GIcA EC 4.1.2.14)) and 2-keto-3-deoxy-6-phospho-D-galactonate aldolases (GalA EC4.1.2.21). 

Addition of Pyruvate to Aldehydes Catalyzed by 2-Keto-3-deoxy-6-phospho-D-gluconate (GIcA), 2-Keto 3- deoxy-6-phospho-D-galactonate aldolases (GalA), and Macrophomate Synthase (MPS)... 

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