Gloves, reusing

Ethylene dibromide is a suspected human carcinogen and worker exposure by all routes should be carefiiUy controUed to levels as low as reasonably achievable (67). Ethylene dibromide causes severe blistering of the skin if contact is prolonged. Eye contact with the Hquid will cause pain, irritation, and temporary impairment of vision. Recommended safety equipment includes safety goggles, a NIOSH approved canister-type gas mask for organic vapors, neoprene gloves, and neoprene overshoes. In case of contact with ethylene dibromide, contaminated clothing and shoes should be removed and eyes or skin washed with plenty of water for at least 15 minutes. Contaminated clothes should be washed before reuse and contaminated shoes should be discarded. 

In all procedures prior to hybridization, utmost precautions must be taken to prevent the contamination of ribonucleases, which results in the degradation of cellular RNA. Gloves must be worn throughout the steps. PBS and water must be autoclaved and, if possible, should be treated with DEPC (trrNote 4). All containers must be clean and ribonuclease-free. Particularly, it is important that, if reused, 12-well plates and insert buckets are treated with HjOj and DEPC (rrrNote 5). 

Wear butyl rubber gloves, laboratory coat, and eye protection. Work in the fume hood from behind safety shield. Add azide to concentrated hydrochloric acid (100 mL acid/g azide). While stirring, add granular tin (2.5 g/g azide). Allow the mixture to stir for 3 hours. Remove the unused tin by filtration and pour the filtrate into a pail of cold water. Neutralize the solution with sodium carbonate and wash into the drain. Discard or reuse recovered tin.8... 

Metal Powder Wear nitrile rubber gloves, laboratory coat, and eye protection. Avoid breathing dust. Scoop into container for reuse or recycling. 

To remove peroxides, wear butyl rubber gloves, laboratory coat, and eye protection. Pour the dioxane (100 mL) into a separatory funnel and shake with a freshly prepared 50% aqueous solution of sodium metabisulfite (20 mL) for 3 minutes. Release the pressure in the funnel at 10-second intervals. Separate the aqueous layer. Retest the dioxane for the continued presence of small amounts of dialkyl peroxides that are not reduced by the metabisulfite treatment. If peroxides are absent, the dioxane can be dried for reuse or packaged for disposal by burning. If peroxides are still present, in the fume hood, place the ether in a 250-mL round-bottom flask equipped with a condenser, and add a solution of 100 mg of potassium iodide in 5 mL of glacial acetic acid and 1 drop of concentrated hydrochloric acid. Reflux gently for 1 hour. Package the ether for disposal by burning.13... 

Keep stock of all azides very low. Stamp date and receipt on package. Wear butyl rubber gloves, eye protection, and laboratory coat. In the fume hood and behind a safety shield, add the azide to 6 M hydrochloric acid (cautiously add a volume of concentrated acid to an equal volume of cold water 150 mL for each 1 g of azide). While stirring, add granular tin (10 g for each 1 g of azide). Continue stirring at room temperature for 30 minutes. Decant or filter the solution into a pail of cold water. Neutralize with sodium carbonate and pour into the drain with at least 50 times its volume of water. Reuse the excess tin or discard as normal refuse.2... 

Use. It is important to wear gloves and safety glasses and to work in a fume hood If you do not have all of these items for safe use of HF, do not use HF as a cleaning material Pour the diluted HF from its plastic storage container into a plastic soaking tank (like a polypropylene beaker). Let the glassware soak in the HF for anywhere from 30 seconds to two minutes. Rinse for at least one minute (or five full flushes) with water. Then rinse with distilled or deionized water. The HF can be reused many times until a decrease in effectiveness is obvious. 

Wear protective gloves/clothing as specified by the manufacturer/supplier or the competent authority. IF ON SKIN Wash with plenty of soap and water. Call a POISON CENTER or doctor/physician if you feel unwell. Specific measures (see. .. on this label). .. reference to supplemental first aid instruction. if measures such as specific cleansing agent is advised. Wash contaminated clothing before reuse. Dispose of contents/container to.... .. (in accordance with local/regional/national/intemational regulation). 

Butylene Oxide Clean protective clothing rubber gloves chemical worker s goggles self-contained breathing apparatus. If any ill effects occur, immediately remove person to fresh air and get medical help if breathing stops, start artificial respiration. Promptly flush with plenty of water remove all contaminated clothing and wash before reuse. Promptly flush with plenty of water for 15 minutes and get medical help. 

Carbon Tetrachloride Organic vapor canister with full face mask protective clothing rubber gloves. Immediately remove to fresh air, keep patient warm and quiet and get medical attention promptly. Start artificial respiration if breathing stops. Flush with plenty of water. Remove contaminated clothing and wash before reuse. Hush with plenty of water for eyes get medical attention. 

Cresols Organic vapor canister unit rubber gloves chemical safety goggles face shield coveralls and/or rubber apron rubber shoes or boots. Remove to fresh air. Flush immediately with plenty of water for at least 15 minutes remove contaminated clothing immediately and wash before reuse discard contaminated shoes. Flush immediately with plenty of water for at least 15 minutes. 

O-Dichlo- robenzene Organic vapor-acid gas respirator neoprene or vinyl gloves chemical safety spectacles, face shield, rubber footwear, apron, protective clothing. Remove victim to fresh air, keep him quiet and warm, and call a physician promptly. Hush with plenty of water get medical attention for eyes remove contaminated clothing and wash before reuse. Rush with plenty of water get medical attention for eyes remove contaminated clothing and wash before reuse. 

Diisoprpo- anolamine Full face mask or amine vapor mask only if required clean, body covering clothing, rubber gloves, apron boots and face shield. If ill effects occur, remove person to fresh air and get medical help. Immediately flush with plenty of water for at least 15 minutes. For eyes, get medical help promptly. Remove and wash contaminated clothing before reuse. Immediately flush with plenty of water for at least 15 minutes. For eyes, get medical help promptly. Remove and wash contaminated clothing before reuse. 

Disposable gloves should be used only once and should not be washed for reuse. 

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