7-Globulins sedimentation

In 1943 Moore and associates (251) reported sedimentation constants for Bence-Jones proteins obtained from the urine of four cases of multiple myeloma, S20 = 2.8 S, 3.4 S, 3.4 S, and 3.4 S, respectively. The sedimentation constant of the electrophoretically separated main abnormal component of the serum proteins was also determined in six cases of multiple myeloma. In cases 1 and 39 of Table VII (page 197), the chief components in both instances were 7-globulins with Sao = 7.1 S. In cases 40 and 42 the chief components with electrophoretic mobilities between /5- and 7-globulins had sedimentation constants S20 = 6.5 S and 7.0 S, respectively. These four abnormal serum proteins clearly were not Bence-Jones proteins. The abnormal component in case 42 was studied further by Shapiro, Ross, and Moore (319), who found it to be a very viscous protein, molecular weight about 160,000. In cases 11 and 41 (the chief component of the first with an electrophoretic mobility between 3- and 7-globulins and of the second corresponding with 3-globulins) sedimentation constants of 820 = 4.0 S and 3.0 S, respectively, were... 

Primary blood components iaclude plasma, red blood cells (erythrocytes), white blood cells (leukocytes), platelets (thrombocytes), and stem cells. Plasma consists of water dissolved proteias, ie, fibrinogen, albumins, and globulins coagulation factors and nutrients. The principal plasma-derived blood products are siagle-donor plasma (SDP), produced by sedimentation from whole blood donations fresh frozen plasma (FFP), collected both by apheresis and from whole blood collections cryoprecipitate, produced by cryoprecipitation of FFP albumin, collected through apheresis and coagulation factors, produced by fractionation from FFP and by apheresis (see Fractionation, blood-plasma fractionation). 

Protein Composition of Milk. Skim milk is a colloidal suspension of extreme complexity. The particulate phase, the casein micelles, consists primarily of a mixture of asi, as2, / , and x-caseins combined with calcium ions and an amorphous calcium-phosphate-citrate complex. The soluble phase contains lactose, a fraction of the caseins and calcium, and, in raw milk, the whey proteins, which are predominantly /3-lacto-globulin and a-lactalbumin. When milk is centrifuged at high speed (in our experiments, 30 min at 110,000 X gravity), the casein micelles sediment. This permits one to separate the two physical phases of skim milk and to measure changes in composition of the phases resulting from... 

Chronic active hepatitis is associated with a strong elevation of IgG with normal or slightly elevated IgM and IgA. Marked elevations of IgG and IgA — with a strong parallel rise in the blood sedimentation rate (BSR), in y-globulin values and in total protein — are... 

Osmotic nephrosis Mannitol, immune globulin, dextrans, hetastarch Urine sediment shows vacuole containing cells... 

The introduction of succinyl residues producing short-range repulsive forces in place of possible short-range attractive forces in the native molecule resulted in a change of hydrodynamic properties of bovine serum albumin (BSA), bovine y-globulin, and /3-lactoglobulin [3], The succinylated derivatives showed markedly increased intrinsic viscosity and Stokes radius and a decrease of sedimentation coefficient [36,37], These results are compatible only with a considerable increase in the effective volume occupied by the succinylated protein molecule compared to its unreacted counterpart. 

Koshiyama, I. Chromatographic and sedimentation behavior of a purified 7s protein in soybean globulins. Cereal Chem. 1968b, 45, 405-412. 

The last component of the complement attack sequence, C9, is a 79,000 molecular weight a-globulin with a sedimentation coefficient of 4.5 S (Hadding and Miiller-Eberhard, 1969). It is composed of a single polypeptide chain (Kolb and Miiller-Eberhard, 1915b) and appears to act by adsorbing to bound C8 (Kolb and Miiller-Eberhard, 1974). 

On the addition of oxalate, blood separates into two layers. A dark red layer contains the blood corpuscles and a yellowish layer contains the plasma and plasma proteins. The plasma proteins can then be separated by fractional precipitation with ammonium sulfate solution. A 20%-25% ammonium sulfate solution will precipitate fibrinogens a 33% solution, globulin and a 50% solution, pseudoglobulin while albumins only precipitate at very high ammonium sulfate concentration. Ultracentrifuge measurements enable four components (X, A, G, M) with different sedimentation coefficients to be differentiated and isolated ... 

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