Globulin coagulation

Primary blood components iaclude plasma, red blood cells (erythrocytes), white blood cells (leukocytes), platelets (thrombocytes), and stem cells. Plasma consists of water dissolved proteias, ie, fibrinogen, albumins, and globulins coagulation factors and nutrients. The principal plasma-derived blood products are siagle-donor plasma (SDP), produced by sedimentation from whole blood donations fresh frozen plasma (FFP), collected both by apheresis and from whole blood collections cryoprecipitate, produced by cryoprecipitation of FFP albumin, collected through apheresis and coagulation factors, produced by fractionation from FFP and by apheresis (see Fractionation, blood-plasma fractionation). 

Assessment of synthetic function serum ALAT, ASAT, ornithine carbamyl transferase, albumin globulin, coagulation profile, prothrombin time, partial thromboplastin time, radioactive amino acids, electrophoresis techniques for serum proteins Assessment of excretion function gamma-glutyml transferase, LDH, bilirubin, alkaline phosphatase... 

The table also lists important globulins in blood plasma, with their mass and function. The a- and p-globulins are involved in the transport of lipids (lipoproteins see p. 278), hormones, vitamins, and metal ions. In addition, they provide coagulation factors, protease inhibitors, and the proteins of the complement system (see p. 298). Soluble antibodies (immunoglobulins see p. 300) make up the y-globulin fraction. 

The surfactants associate at the oxide surface to form hemi-micelles with their hydrophobic groups exposed to the aqueous phase at low concentrations, but at higher concentrations, with the hydrophilic groups turned outwards. Hematite coated with various proteins (ovalbumin, y-globulin, lysozyme) adopted either the iep of the proteins or a value between that of the oxide and the protein and displayed modified coagulation behaviour (Johnson and Matijevic, 1992). 

Immunodeficiency 100-200 mg/kg/mo IV at 0.01-0.04 mL/kg/min to 400 mg/kg/dose max UP 400 mg/kg/dose IV daily x 5 d BMP 500 mg/kg/wk X in renal insuff Caution [C, ] Separate administration of live vaccines by 3 mo Contra IgA deficiency w/ Abs to IgA, severe thrombocytopenia or coagulation disorders Disp Inj SE Associated mostly w/ inf rate GI upset Interactions X Effects OF live virus vaccines EMS May cause anaphylactic Rxn OD Unlikely Immune Globulin, Subcutaneous (Vivaglobin) [Immune Serum] Uses Primary immunodeficiency Action IgG supl Dose 100—200 mg/kg BW subq wkly abd, thighs, upp arms, or lat al hip Caution [C, ] Contra Hx anaphylaxis to immune globulin some IGA deficiency Disp Inj SE Inj site Rxns, HA, GI complaint, fevCT, N, D, rash, sore throat EMS May be self administered at home may cause anaphylactic Rxn OD Unlikely to cause life-threatening Sxs... 

Table 3-7 Heat Coagulation Temperatures of Some Albumins and Globulins and Casein...

The effect of NaCS on all coagulation factors (I—XII) is at least the same as that of heparin. In fact NaCS inhibits the action of the anticoagulant factor VIII (anti-thermophilic globulin) much more effectively than heparin. Therefore, it may be concluded that NaCS acts mainly on factor VIII and partly on factor IX (plasma thromboplastic component) the latter was found to be suppressed by heparin. 

Antithrombin III is formed in the hepatocytes as an U2-globulin. It serves as a physiological inhibitor of serin proteases in the coagulation system and thus inhibits activating factors Ila, IXa, Xa, XIa and Xna. As the name implies, AT III is most effective as an inhibitor of thrombin. Half-life is 2-3 days. 

Globulins are insoluble or sparingly soluble in water, but their solubility is greatly increased by the addition of neutral salts such as sodium chloride. These proteins are coagulated by heat. They are deficient in methionine. Example -Serum globulin. Fibrinogen, Myosin of muscle and Globulins of pulses. 

Comenzo RL, Malachowski ME, Meissner HC, Fulton DR, Berkman EM. Immune hemolysis, disseminated intravascular coagulation, and serum sickness after large doses of immune globulin given intravenously for Kawasaki disease. J Pediatr 1992 120(6) 926-8. 

Within the two broad classes, proteins are subdivided on the basis of physical properties, especially solubility for example, albumins (soluble in water, coagulated by heat), globulins (insoluble in water, soluble in dilute salt solutions), etc. 

Group Specific Media Various Nucleotide-requiring enzymes, coagulation factors, DNA binding proteins, a2-macro-globulin HiTrap Blue 17-0412-01 17-0413-01 5 x 1 ml 1 x 5 ml HSA 20 mg/ml... 

Procedures for the direct weighing of precipitated or heat-coagulated protein of serum (D8) are uneconomic. Turbidity methods have been discussed in relation to total globulin, and near- and far-ultraviolet spectrophotometry in relation to albumin. Of these, spectrophotometry at about 215 nm is the method of choice. A simple and direct fluorimetric method (K33) should also prove useful. BPB staining of serum on paper, after protein concentration by the Weiss ring-oven technique, has been tried. This is a rapid technique (F3), but the color, confined as it is to a line, is difficult to quantitate, so that the method is insensitive and unsatisfactory in its present form. 

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