Gene subtraction

Matsumoto, N, Kitayama, H, Kitada, M, Kimura, K, Noda, M, and Ide, C, 2003. Isolation of a set of genes expressed in the choroid plexus of the mouse using suppression subtractive hybridization. Neuroscience 117,405-415. 

Kuang TO et al. Differential screening and suppression subtractive hybridisation identified genes differentially expressed in an estrogen receptor-positive breast carcinoma cell line. Nucleic Acids Res 1998 26 1116-1123. 

Villaret DB et al. Identification of genes overexpressed in head and neck squamous cell carcinoma using a combination of complementary DNA subtraction and microarray analysis. Laryngoscope 2000 11 374-381. 

Wang XB et al. Subtracted differential display genes with amphetamine-altered expression patterns include calcineurin. Mol Brain Res 1998 53 344-347. 

Figure 3.11 Differential pulse voltammograms (DPVs) for guanine at bare glassy carbon, SWNT modified glassy carbon and bamboo-modified glassy carbon. DNA cone, 0.4 mgmL (b) the corresponding DPV plots observed in (a) but with background subtraction. The signal gene rated from...

The characterisation of memory-related genes and proteins belongs to the hot spots of current memory research (D Agata and Cavallaro 2002). Respective studies in the field of aversive memories employ different molecular biological methods including in situ hybridisation (Ressler et al. 2002), differential display (Huang et al. 1998), subtractive hybridisation (Stork et al. 2001) and DNA microarrays (Kida et al. 2002). Most critical for the correct interpretation... 

Efforts to elucidate the genetic basis of biosynthetic pathways tend to result in the identification of structural genes, i.e. genes encoding enzymes that catalyze the conversion of intermediates in a specific pathway. This is in part due to the relatively high expression of the structural genes, which results in abundant representation in expressed sequence tag (EST) libraries, or high probability of identification when cDNA subtraction methods or other methods that rely on differential expression are used. 

Suppression-subtractive hybridization (SSH) is another hybridization strategy that is being pursued to examine differential gene expression following parasite infection in the snail host. At least two major laboratories are currently engaged in using this approach towards the isolation of genes that are specifically expressed in either resistant or susceptible snails in response to infection. Initial results from these studies showed differential expression of several transcripts in a resistant (BS-90) and susceptible snail (M-line) in response to... 

---