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In situ hybridisation

Wagner, M. Horn, M. Daims, H. Fluorescence in situ hybridisation for the identification and characterisation of prokaryotes. Curr. Opin. Microbiol. 2003, 6, 302-309. [Pg.18]

Keith D. Miller, Chief Scientific Officer, UCL-Advanced Diagnostics, Cancer Institute, Director of the UK National External Quality Assessment Scheme for Immunocytochemistry In-situ Hybridisation and, Fellow of the Institute of Biomedical Science, London, UK... [Pg.468]

Corporeau, C. and Auffret, M., In situ hybridisation for flow cytometry a molecular method for monitoring stress-gene expression in hemolymph cells of oysters, Aquatic Toxicol., 64, 427, 2003. [Pg.383]

Abstract In situ hybridisation is a powerful technique for determining the distribution of specific mRNA species in tissues such as brain that comprise different cell types. Its utility in molecular neurobiology has driven the development of a variety of variations of this technique will be reviewed. [Pg.364]

The characterisation of memory-related genes and proteins belongs to the hot spots of current memory research (D Agata and Cavallaro 2002). Respective studies in the field of aversive memories employ different molecular biological methods including in situ hybridisation (Ressler et al. 2002), differential display (Huang et al. 1998), subtractive hybridisation (Stork et al. 2001) and DNA microarrays (Kida et al. 2002). Most critical for the correct interpretation... [Pg.17]

Fig. 2. In situ localisation of rabl6 mRNA in developing wheat seeds. A, Scanning electron micrograph of scutellar/starchy endosperm boundary. DC, depleted cells SE, scutellar epithelial cells. B, Dark field micrograph of in situ hybridised rabl6 sense RNA probe. No specific hybridisation is visible. C, Dark field micrograph of in situ hybridised rabl6 antisense RNA probe. Note specific hybridisation to depleted cells. Scanning electron microscopy was performed according to Mundy et al. (1986) in situ hybridisation after Raikhel et al. (1989). Fig. 2. In situ localisation of rabl6 mRNA in developing wheat seeds. A, Scanning electron micrograph of scutellar/starchy endosperm boundary. DC, depleted cells SE, scutellar epithelial cells. B, Dark field micrograph of in situ hybridised rabl6 sense RNA probe. No specific hybridisation is visible. C, Dark field micrograph of in situ hybridised rabl6 antisense RNA probe. Note specific hybridisation to depleted cells. Scanning electron microscopy was performed according to Mundy et al. (1986) in situ hybridisation after Raikhel et al. (1989).
In situ hybridisation application to developmental biology and medicine. Edited by N. Harris and D.G. Wilkinson... [Pg.314]

In situ hybridisation Localizes specific genes within cells with nucleic acid-labelled probes Sensitivity and specificity dependent upon tissue and probe quality Yes... [Pg.422]

Jackson. P., Dockey, D. A. Lewis, F. A., and Wells, M. (1990) Applications of lnm gold probes on paraffin sections for in situ hybridisation histochemistry. J. Clin. Pathol. 43, 810-812. [Pg.102]

Haygood, M. G. and Davidson, S. K., Small-subunit rRNA genes and in situ hybridisation with oligonucleotides specific for the bacterial symbionts in the larvae of the bryozoan Bugula neritina and proposal of Candidatus endobugula sertula, Appl. Env. Microbiol., 63, 4612, 1997. [Pg.102]

Chalmers DT, Watson SJ. Comparative anatomical distribution of 5-HT1A receptor mRNA and 5-HT1A binding in rat brain a combined in situ hybridisation/in vitro receptor autoradiographic study. Brain Res 1991 561 51-60. [Pg.349]

Ward RP, Hamblin MW, Lachowicz JE, Hoffman BJ, Dibley DR, Dorsat DM. Localization of serotonin subtype 6 receptor messenger RNA in the rat brain by in situ hybridisation histochemistry, Neuroscience 1995 64 1105-1111. [Pg.508]

Di Palma S, Collins N, Faulkes C, Ping B, Ferns G, Haagsma B, et al. Chromogenic in situ hybridisation (CISH) should be an accepted method in the routine diagnostic evaluation of HER2 status in breast cancer. J Clin Pathol 2007 60(9) 1067-8. [Pg.100]

Saez A, Andreu FJ, Segui MA, Bare ML, Fernandez S, Dinares C, et al. HER-2 gene amplification by chromogenic in situ hybridisation (CISH) compared with fluorescence in situ hybridisation (FISH) in breast cancer-A study of two hundred cases. Breast 2006 15(4) 519-27. [Pg.100]

Di Palma S, Collins N, Bilous M, Sapino A, Mottolese M, KapranosN.etal. A quality assurance exercise to evaluate the accuracy and reproducibility of chromogenic in situ hybridisation for HER2 analysis in breast cancer. J Clin Pathol 2008 61(6) 757-60. [Pg.101]

Arnould L, Denoux Y, MacGrogan G, Penault-Llorca F, Fiche M, Treilleux I, et al. Agreement between chromogenic in situ hybridisation (CISH) and FISH in the determination of HER2 status in breast cancer. Br J Cancer 2003 88(10) 1587-91. [Pg.101]

R. Erhadt, D. Bradford, R. J. Sevior, R. Amann and L. L. Blackall (1997). Development and use of fluorescent in situ hybridisation probes for the detection and identification of Microtrix parvicella in activated sludge. Syst. Appl. Microbiol., 20, 310-318. [Pg.223]

Harrington KA, Augood SJ, Faull RL, McKenna PJ, Emson PC. 1995. Dopamine D1 receptor, D2 receptor, proenkephalin A and substance P gene expression in the caudate nucleus of control and schizophrenic tissue A quantitative cellular in situ hybridisation study. Brain Res Mol Brain Res 33 333-342. [Pg.481]

The spectral karyotype fluorescence in situ hybridisation (FISH)... [Pg.278]

Bums,J., Graham, A. K., Frank, C., Fleming, K. A., Evans, M. P., and McGee,J. O D. (1987) Detection of low copy human papilloma virus DNA and mRNA in routine paraffin sections of cervix by nonisotopic in situ hybridisation./. CUn. Pathol. 40, 858-864. [Pg.429]

Eller G., Stubner S., and Erenzel P. (2001) Group-specific 16S rRNA targeted probes for the detection of type 1 and type 11 methanotrophs by fluorescence in situ hybridisation. FEMS Microbiol. Lett. 198, 91-97. [Pg.4264]

In situ hybridisation revealed the distribution of dopamine D2 receptor mRNA in the rat brain. High abundance was found in regions which are classically associated with dopaminergic neurotransmission, including the caudate putamen, nucleus accumbens, olfactory tubercle,... [Pg.8]

Holzenberger, M. and Lapointe, F. Expression of insulin-like growth factor-I (IGF-I) and IGF-II in the avian brain relationship of in situ hybridisation patterns with IG F type 1 receptor expression. Int. J. Dev. Neurosci., 2000, 18, 69—82. [Pg.115]

Bridges D, Rice AS, Egertova M, Elphick MR, Winter J, Michael GJ (2003) Localisation of cannabinoid receptor 1 in rat dorsal root ganglion using in situ hybridisation and immunohistochemistry. Neuroscience 119 803-812... [Pg.138]

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Hybridisation

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