Fungal identification

Davenport, R.R. (1995a) Forensic investigation for yeasts. European Commission Science Research Development, Proceedings of Meeting on Fungal Identification Techniques, Barcelona, Spain. 

Although much data has been collated [3], such infonnation illustrates that fungal identification based on fungal morphology requires considerable experience and that it is all too easy to mis-identify specific fungi. It is possible to mis-identify poisonous fungi for those listed here and hence this approach should only really be undertaken by an expert, or under expert guidance. 

Morgan, A., Boddy, L., Mordue, J.E.M. and Morris, C.W. (1998) Evaluation of artificial neural networks for fungal identification, employing morphometric data from spores of Pestalotiopsis species. Mycological Research, 102 975-984. 

McMillan DC, PP Fu, CE Cerniglia (1987) Stereoselective fungal metabolism of 7,12-dimethylbenz[a]anthra cene identification and enantiomeric resolution of a K-region dihydrodiol. Appl Environ Microbiol 53 2560-2566. 

R. Sanders, M. Alt, K, Groppe, T. Boiler, and A. Wiemken, Identification of ribosomal DNA polymorphisms among and within spores of the Glomules application to studies on the genetic diversity of arbuscular mycorrhizal fungal communities. New Phytol. /30 419 (1995). 

Maquelin, K. Kirschner, C. Choo-Smith, L. P. Ngo-Thi, N. A. van Vreeswijk,T. Stammler, M. Endtz, H. P. Bruining, H. A. Naumann, D. Puppels, G. J. Prospective study of the performance of vibrational spectroscopies for rapid identification of bacterial and fungal pathogens recovered from blood cultures. J. Clin. Microbiol. 2003,41, 324-329. 

The fungal transformation of estrogens, by enzymes or cells, seems to produce metabolites with reduced estrogenic activity in short periods, usually days or even few hours. However, only oligomeric products have been identified in enzymatic processes and a lack in the identification of metabolites derived from whole cell fungal systems is still observed. 

The instrumental analysis for the identification of UV filters degradation products formed during the fungal treatment process was performed by means of HPLC coupled to tandem mass spectrometry using a hybrid quadrupole-time-of-flight mass spectrometer (HPLC-QqTOF-MS/MS). Chromatographic separation was achieved on a Hibar Purospher STAR HR R-18 ec. (50 mm x 2.0 mm, 5 pm, from Merck). In the optimized method, the mobile phase consisted of a mixture of HPLC grade water and acetonitrile, both with 0.15% formic acid. The injection volume was set to 10 pL and the mobile phase flow-rate to 0.3 mL/min. 

Therefore, mRNA capping represents a potentially useful target for inhibitors with antifungal or antiviral activity indeed, whole-yeast cell-based assays developed to identify and characterize inhibitors of fungal mRNA capping allowed the identification and characterization of... 

Shastry, M., Nielsen, J., Ku, T., Hsu, M. J., Liberator, P., Anderson, J., Schmatz, D., and Justice, M. C. (2001). Species-specific inhibition of fungal protein synthesis by sordarin Identification of a sordarin-specificity region in eukaryotic elongation factor 2. Microbiology 147, 383-390. 

Komillowicz-Kowalska TK, Wrzosek M, Ginalska G, Iglik H, Bancerz R (2006) Identification and application of a new fungal strain Bjerkandera audusta R59 in decolorization of daunomycin wastes. Enz Microbial Technol 38 583-590... 

Jalal MAF, van der Helm D (1991) Isolation and Spectroscopic Identification of Fungal Siderophores. In Winkelmann G (ed) Handbook of Microbial Iron Chelates. CRC, Boca... 

Studies continue to be published in which only the results of bioassay of fungal extracts are presented without the identification of active compounds. Some of these studies suggest significant activities for the compounds contained in the extracts. Such studies represent opportunities for the natural products chemist to collaborate with the biology- and molecular biology-oriented groups for more complete studies of the plants and associated endophytic fungi. 

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