Fungal protein

In terms of amino acids bacterial protein is similar to fish protein. The yeast s protein is almost identical to soya protein fungal protein is lower than yeast protein. In addition, SCP is deficient in amino acids with a sulphur bridge, such as cystine, cysteine and methionine. SCP as a food may require supplements of cysteine and methionine whereas they have high levels of lysine vitamins and other amino acids. The vitamins of microorganisms are primarily of the B type. Vitamin B12 occurs mostly hi bacteria, whereas algae are usually rich in vitamin A. The most common vitamins in SCP are thiamine, riboflavin, niacin, pyridoxine, pantothenic acid, choline, folic acid, inositol, biotin, B12 and P-aminobenzoic acid. Table 14.4 shows the essential amino acid analysis of SCP compared with several sources of protein. 

Dominguez, J. M., Gomez-Lorenzo, M. G., and Martin, J. J. (1999). Sordarin inhibits fungal protein synthesis by blocking translocation differently to fusidic arid. j. Biol. Chem. 274, 22423-22427. 

Shastry, M., Nielsen, J., Ku, T., Hsu, M. J., Liberator, P., Anderson, J., Schmatz, D., and Justice, M. C. (2001). Species-specific inhibition of fungal protein synthesis by sordarin Identification of a sordarin-specificity region in eukaryotic elongation factor 2. Microbiology 147, 383-390. 

Tobacco petiole and stem Translocation of fungal protein in plants Taylor et al. (24)... 

Taylor R, Bailey BA, Dean JFD, Anderson JD. Translocation of fungal protein in plants, in Tissue Printing Tools for the Study of Anatomy, Histochemistry, and Gene Expression (ReidP, Pont-Lezica R, del Campillo E, Taylor R, eds.), Academic Press, New York, 1992, pp. 43-58. 

M Sadler. Fungal protein. In B Hudson, ed. New and Developing Sources of Food Proteins. London Chapman Hall, 1994, pp 343-360. 

Monti F, Ripamonti F, Hawser SP, Islam K (1999) Aspirochlorine A Highly Selective and Potent Inhibitor of Fungal Protein Synthesis. J Antibiot 52 311... 

Isoelectric focusing and electrophoresis were used for protein mapping and to study protein extraction in horizontal ultra-thin-layer format [138], The 0.12-0.36-mm-thick polyacrylamide gel layer was deposited onto tiny glass plates (e.g., microscope slides). The method enabled the analysis of 1-ng tissue culture specimens. Ultra-thin-layer polyacrylamide isoelectric focusing gel was also employed in two-dimensional analysis of plant and fungal proteins. Marlow et al. [139] reported on the use of 0.2-mm semirigid backing (polyester)-supported... 

The CdS nanoparticles were autocapped by the fungal proteins, which were responsible for imparting them high stability. The colloidal solution of as-prepared CdS nanoparticles was extremely stable with no evidence for aggregation even after 10 months of continuously running the column. The long-term stability of the as-prepared CdS... 

The Kishi strategy was used to construct the 7-mem be red disulfide bridge of the analogue 40.3 of Aspirochlorine [40.4, Scheme 5.40], an Aspergillus metabolite which is a selective and potent inhibitor of fungal protein synthesis.76... 

Figure 21. Affinity chromatography of fungal proteins on columns packed with sorbent A) vanillin chemically bonded with the CPG surface B) vanillin chemically bonded with the boron-enriched CPG surface. Peroxidase activity-open rings absorbance at 280 nm-black rings.

The activity of blasticidin S involves primarily the inhibition of fungal protein synthesis. In the presence of blasticidin S, the incorporation of amino acids into protein is reduced by 80-90% (Gottlieb et al., 19SS Misato et al., 1%1 Huang et al.. 1964a). It was foimd that blasticidin S reduces the formation of aminoacyl-tRNA. Hence the next step, the incorporation of the amino acid, is also block. The site of primary action is not yet known. 

Laukevics JJ, Apsite AF, Viesturs UE (1984) Solid substrate fermentation of wheat straw to fungal protein. Biotechnol Bioeng 26 1465-1474... 

Currently, the compound 217 is the only available antimetabolite drug having antifungal activity. It inhibits fungal protein synthesis by replacing 187 with 177 in fungal RNA. 217 also inhibits thymidylate synthetase via 5-fluorodeoxyuridine monophosphate and thus interferes with fungal DNA synthesis. 

Driver, J., Hamilton, M., Rillig, M., 2003. The secreted AM fungal protein glomalin binds strontium in solution. Book of Abstracts of the Fourth International Conference on Mycorrhizae ICOM4, Montreal, Canada, 10-15 August 2003, p. 225. 

Orchard MG, Neuss JC, Galley CM, Carr A, Porter DW, Smith P, Scopes DI, Haydon D, Vousden K, Stubberfield CR, Young K, Page M. Rhodanine-3-acetic acid derivatives as inhibitors of fungal protein mannosyl transferase 1 (PMTl). Bioorg. Med. Chem. Lett. 2004,14, 3975-3978. 

Another study focused on PDMS surfaces made hydrophilic by modification with hydrophobins (small, cysteine-rich and amphiphilic fungal proteins). This approach was used to pattern antigen molecules, followed by immunoassays. For example, chicken immunoglobulin G was found to be compatible with the hydrophhobin-modified PDMS. 

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