Fluorescence spectrometry with high-performance liquid

K. K. Chee, M. K. Wong, H. K. Lee, Optimization of microwave-assisted solvent extraction of polycyclic aromatic hydrocarbons in marine sediments using a microwave extraction system with high-performance liquid chromatography-fluorescence detection and gas chromatography-mass spectrometry. Anal. Chim. Acta. 330 (1996), 217. 

Gimeno R.A. et al., 2002. Determination of polycyclic aromatic hydrocarbons and polycyclic aromatic sulfur heterocycles by high-performance liquid chromatography with fluorescence and atmospheric pressure chemical ionization mass spectrometry detection in seawater and sediment samples. J Chromatogr A 958 141. 

In this regard several sophisticated chromatographic methods, with a quantification limit down to about 0.2 ng/g, have been developed and published for the determination of zearalenone. The methods were mainly based on high-performance liquid chromatography (HPLC) with fluorescence detection (Krska 1998 Visconti and Pascale 1998 Schuhmacher et al. 1998 Tanaka et al. 2000), but HPLC with mass spectrometry detection was also used (Shirai et al. 2000 Josephs et al. 2001). 

As seen in Chapter 9.C.2, a very wide variety of organics are found in particles in ambient air and in laboratory model systems. The most common means of identification and measurement of these species is mass spectrometiy (MS), combined with either thermal separation or solvent extraction and gas chromatographic separation combined with mass spectrometry and/or flame ionization detection. For larger, low-volatility organics, high-performance liquid chromatography (HPLC) is used, combined with various detectors such as absorption, fluorescence, and mass spectrometry. For applications of HPLC to the separation, detection, and measurement of polycyclic aromatic hydrocarbons, see Wingen et al. (1998) and references therein. 

Figure 6.1 Bar-graph of MeHg in CRM 580. The results correspond to six replicate determinations as performed by different laboratories using various methods. MEANS indicates the mean of laboratory means with 95% confidence interval. Abbreviations-. CVAAS, cold vapour atomic absorption spectrometry CVAFS, cold vapour atomic fluorescence spectrometry ECD, electron capture detection GC, gas chromatography HPLC, high-performance liquid chromatography ICPMS, inductively coupled plasma mass spectrometry MIP, microwave induced plasma atomic emission spectrometry QFAAS, quartz furnace atomic absorption spectrometry SFE, supercritical fluid extraction.

Fluorescence spectrometry is used in both research and surveillance to help assure both the farmer and the consumer a continued bounty of high quality agricultural products while preserving the quality of the environment. My objective is to discuss some of our current research and to share with you several interesting observations where we have used fluorescence as a monitor in high performance liquid chromatography. 

A large number of analytical methods for detection of CWA degradation products are available. Gas chromatography/mass spectrometry (GC/MS) and high performance liquid chromatography (HPLC) with fluorescence detection are the most widely used.6,7 However, limitations of these methods include complicated derivatization procedures, long analysis time, and difficulty in miniaturization. 

L. N. Liang, G. B. Jiang, J. G. Liu, J. T. Hu, Speciation analysis of mercury in seafood by using high-performance liquid chromatography on-line coupled with cold-vapor atomic fluorescence spectrometry via a post column microwave digestion, Anal. Chim. Acta, Ml (2003), 131-137. 

Modern tHcy methods include enzyme immunoassays and chromatographic-based methods. In practice, immunoassays " are most often used for routine purposes (e.g., fluorescence polarization immunoassay IFPIA] as run on Abbott s IMx and AxSYM platforms) Chromatographic assays include amino acid analysis high-performance liquid chromatography (HPLC) with ultraviolet, fluorescence, or electrochemical detection ° " capillary electrophoresis with fluorescence detection gas chromatography-mass spectrometry (GC-MS) and liquid chromatography with tandem MS (MS-MS). 

Beltran JL, Guiteras J, Ferrer R, Three-way multivariate calibration procedures applied to high-performance liquid chromatography coupled with fast-scanning fluorescence spectrometry detection. determination of polycyclic aromatic hydrocarbons in water samples, Analytical Chemistry, 1998b, 70,1949-1955. 

Gradeen, C.Y. Billay, D.M. Chan, S.C. Analysis of bumetanide in human urine by high-performance liquid chromatography with fluorescence detection and gas chromatography/mass spectrometry. J.Anal.Toxicol., 1990, 14, 123-126... 

Saugy, M. Meuwly, R Munafo, A. Rivier, L. Rapid high-performance liquid chromatographic determination with fluorescence detection of furosemide in human body fluids and its confirmation by gas chromatography-mass spectrometry. J.Chromatogr., 1991, 564, 567-578 [serum urine fluorescence detection warfarin (IS) pharmacokinetics gradient LOD 10 ng/mL]... 

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