Fluorescence photobleaching recovery

Petersen N. O. and Elson E. L. (1986) Measurements of Diffusion and Chemical Kinetics by Fluorescence Photobleaching Recovery and Fluorescence Correlation Spectroscopy Methods Enzymol. 130, 454-84. 

D. Axelrod, D. E. Koppel, J. Schlessmjer, E. Elson, and W. W. Webb, Mobility measurement by analysis of fluorescence photobleaching recovery kinetics, Biophys. J. 16, 1055-1069... 

To increase the speed of the TIRF-based kinetic techniques, the perturbation can be optical rather than chemical. If the evanescent wave intensity is briefly flashed brightly, then some of the fluorophores associated with the surface will be photobleached. Subsequent exchange with unbleached dissolved fluorophores in equilibrium with the surface will lead to a recovery of fluorescence, excited by a continuous but much attenuated evanescent wave. The time course of this recovery is a measure of the desorption kinetic rate k2. This technique1-115) is called TIR/FRAP (or TIR/FPR) in reference to fluorescence recovery after photobleaching (or fluorescence photobleaching recovery). 

T. P. Burghardt and D. Axelrod, Total internal reflection/fluorescence photobleaching recovery study of serum albumin adsorption dynamics, Biophys. J. 33, 455-468 (1981). 

M. M. Timbs and N. L. Thompson, Slow rotational mobilities of antibodies and lipids associated with substrate-supported phospholipid monolayers as measured by polarized fluorescence photobleaching recovery, Biophys. J. 58, 413-428 (1990). 

D. M. Soumpasis, Theorectical-analysis of fluorescence photobleaching recovery experiments, Biophys. J. 41, 95-97 (1983). 

C. Lagerholm, T. E. Starr, Z. N. Volovyk, and N. L. Thompson, Rebinding of IgE Fabs at haptenated planar membranes Measurement by total internal reflection with fluorescence photobleaching recovery, Biochemistry 39, 2042-2051 (2000). 

Yu and Russo 52a reported the fluorescence photobleaching recovery and dynamic light... 

A new technique for measuring equilibrium adsorption/desorption kinetics and surface diffusion of fluorescent-labelled solute molecules at surfaces was developed by Thompson et al.74). The technique combines total internal reflection fluorescence with either fluorescence photobleaching recovery or fluorescence correlation spectroscopy with lasers. For example, fluorescent labelled protein was studied in regard to the surface chemistry of blood 75). 

Figure 10. Mutual diffusion coefficients, of the two molecular populations of a fluorescent dye dispersed in poly(1-trimethyl-l-propyne) plotted as functions of reciprocal absolute temperature. The technique used was laser fluorescence photobleaching recovery.

Barisas, B.G. and M.D. Leuther, Fluorescence photobleaching recovery measurement of protein absolute diffusion constants. Biophysical Chemistry, 1977,10, 221-229. 

W.R. Zipfel, W.W.W. Webb, In vivo Diffusion Measurements Using Multiphoton Excitation Fluorescence Photobleaching Recovery and Fluorescence Correlation Spectroscopy, in A. Periasamy (ed.), Methods in Cellular Imaging, Oxford University Press, 216 (2001)... 

Hagen, G. M., Roess, D. A., and Barisas, B. G. (2006) Fluorescence photobleaching recovery using total internal reflection interference fringes. Anal. Biochem. 356, 30-35. 

Saxton, M.J. Anomalous diffusion due to binding a Monte Carlo smdy. Biophys. J. 70(3), 1250-1262 (1996). http //www.biophysj.org/cgi/content/abstract/70/3/1250 Saxton, M.J. Anomalous subdiffusion in fluorescence photobleaching recovery a Monte Carlo study. Biophys. J. 81(4), 2226-2240 (2001). http //www.biophysj.org/cgi/ content/abstract/81/4/2226... 

The spatial resolution in FRAP (also known as fluorescence photobleaching recovery, FPR) measurements is limited by the minimum diffraction-limited size of the laser beam [24,25]. 

Blonk JCG, Don A, Van Aalst H, Birmingham JJ (1993) Fluorescence photobleaching recovery in the confocal scanning light microscope. J Microsc 169 363-374... 

Andreasen K, Nielsen PH (1997) Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge. Appl Environ Microbiol 63(9) 3662—3668 Arnold FH, Blanch HW, Wilke CR (1985) Analysis of affinity separations II the characterization of affinity columns by pulse techniques. Chem Eng J 30 25-36 Axelrod D, Koppel DE, Schlesinger J, Elson E, Webb WW (1976) Mobility measurement by analysis of fluorescence photobleaching recovery kinetics. Biophys J 16 1055-1069... 

Edwin, N. J. Hammer, R. R McCarley, R. L. Russo, P. S. Reversibility of p-amyloid self-assembly Effects of pH and added salts assessed by fluorescence photobleaching recovery. Biomacromolecules 2010,11, 341-347. 

Corbett, J. D. Cho, M. R. Golan, D. E. Deoxygenation affects fluorescence photobleaching recovery measurements of red cell membrane protein lateral mobility. Biophys. J. 1994,66,25-30. 

---