Fluorescence recovery after photobleaching microscopy

Total internal reflection fluorescence (TIRF) microscopy, fluorescence in situ hybridization (FISH), fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging microscopy (FLIM). 

Ratio TV, Longo ML. Obstructed diffusion in phase-separated 64. supported lipid bilayers a combined atomic force microscopy and fluorescence recovery after photobleaching approach. Biophys. J. 2002 83 3380-3392. 

Biological membranes are not rigid, static structures. On the contrary, lipids and many membrane proteins are constantly in lateral motion, a process called lateral diffusion. The rapid lateral movement of membrane proteins has been visualized by means of fluorescence microscopy through the use of the technique of fluorescence recovery after photobleaching (FRAP Figure 12.29). 

Measurement of transient concentration profiles of deuterated polymers in polymer blends using forward recoil spectroscopy Measurement of transient concentration profiles of fluorescently labeled molecules in capillary tubes using epifluorescence microscopy Measurement of transient concentration profiles of fluorescently labeled molecules in biological tissues using epifluorescence microscopy Fluorescence recovery (or redistribution) after photobleaching (FPR)... 

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