Fluorescence recovery after photobleaching dynamics

The fluorescent spectroscopy is one of the optical techniques that are widely used in the study of structure and dynamic properties of lipids in lamellar phases [76]. The Fluorescence Recovery after Photobleaching (FRAP) is successfully applied in the lateral diffusion studies of BLM (e.g. 77]. FRAP has been employed to study similar phenomena at the air/water interface of Langmuir trough [78]. 

Consider surfaces that are inert and may be made (molecularly) smooth, so that, optically speaking, they may be treated as Fresnel surfaces. Mica, certain polished glasses, quartz and silicon wafer surfaces may belong to this category. For such well-defined systems the optical techniques introduced in sec. 1.7.10 come to mind reflectometry, ellipsometry, and (to study the dynamics) fluorescence recovery after photobleaching (FRAP). The principles of these techniques have been outlined in that section. 

Nagao, I., Aoki, Y., Tanaka, M. and Kinjo, M. (2008) Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching. FEBS J., 275, 341-349. 

Techniques such as forced Rayleigh scattering, pulsed gradient NMR, and fluorescence recovery after photobleaching have recently made information concerning self-diffusion available for polyelectrolyte across the range of solution concentrations. The complex diffusion dynamics imcovered for by dynamic... 

The nature of polymer motion in semidilute and concentrated solutions remains a major question of macromolecular science. Extant models describe polymer dynamics very differently 3-11). Many experimental methods have been used to study polymer dynamics (12). One meAod is probe diffusion, in which inferences about polymer dynamics are made by observing the motions of dilute mesoscopic probe particles diffusing in the polymer solution of interest. Probe diffusion can be observed by several experimental techniques, for example, quasi-elastic light scattering spectroscopy (QELSS), fluorescence recovery after photobleaching (FRAP), and forced Rayleigh scattering (FRS). 

Jourdainne, L., Sn, Lecuyer, Amtz, Y., Picart, C., Schaaf, P., Senger, B., Voegel, J.-C., Lavalle, P., Charitat, T. Dynamics of poly(l-lysine) in hyaluronic acid/poly(l-lysine) multilayer films studied by fluorescence recovery after pattern photobleaching. Langmuir 24, 7842-7847 (2008)... 

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