SCANNING CONFOCAL

Figure Bl.18.11. Confocal scanning microscope in reflection the pinliole in front of the detector is in a conjugate position to the illumination pinliole. This arrangement allows the object to be optically sectioned. The lens is used to focus the light beam onto the sample and onto the pinliole. Thus, the resulting point spread fimctioii is sharpened and the resolution increased.

Cork T and Kino G S 1996 Confocal Scanning Optical Microscopy and Related Imaging Systems (New York Academic) Gu Min 1996 Principles of Three Dimensional Imaging In Confocal Microscopes (Singapore World Scientific)... 

Ling X, Pritzker M D, Byerley J J and Burns C M 1998 Confocal scanning laser microscopy of polymer coatings J. Appl. Polym. Sc/. 67 149-58... 

Leonas K K 1998 Confocal scanning laser microscopy a method to evaluate textile structures Am. Dyest. Rep. 87 15-18 Wilson K R ef a/1998 New ways to observe and control dynamics Proc. SPIE 3273 214-18... 

Diaspro A, Chirico G, Federici F, Cannone F, Beretta S, Robello M (2001) Two-photon microscopy and spectroscopy based on a compact confocal scanning head. J Biomed Opt 6 300-310... 

Bohmer, M., Pampaloni, F., Wahl, M., Rahn, H. J., Erdmann, R. and Enderlein, J. (2001). Time-resolved confocal scanning device for ultrasensitive fluorescence detection. Rev. Sci. Instrum. 72, 4145-52. 

In this chapter, it was shown that filterFRET is an easy, intuitive and quantitative alternative to record sensitized emission and FRET efficiency. The major advantages of filterFRET over donor-based FRET detection methods (FLIM) are that it can be carried out with standard wide-held or confocal fluorescence microscopes that are available in most laboratories, and that it yields additional data on the acceptor population. FilterFRET is also fast, requiring just two confocal scans (if need be on a line-by-line basis) which minimizes the risk of artifacts due to, for example, organelle movement in living cells, and acquisition can be optimized for each channel independently. However, quantitative... 

Verhaegh, N.A.M., and van Blaaderen, A. (1994) Dispersions of rhodamine-labeled silica spheres synthesis, characterization, and fluorescence confocal scanning laser microscopy. Langmuir 10, 1427-1438. 

MJ. Schermer, Confocal scanning microscopy in microarray detection, in DNA Microarrays A Practical Approach (M. Schena, ed.), p. 17, Oxford University Press (1999). 

Confocal scanning Examination of cells in Cryoelectron Imaging of biological macromolecules in the... 

Shooton, D. (ed.) (1993) Electronic Light Microscopy. The Principles and Practice of Video-Enhanced Contrast, Digital Intensified Fluorescence, and Confocal Scanning Light Microscopy. Wiley-Liss, New York. 

Brakenhoff, G. J., Blom, P., and Barends, P. (1979) Confocal scanning light microscopy with high aperture immersion lenses. J. Micros. 117, 219-232. 

M., and Nanninga, N. (1985) Three-dimensional chromatin distribution in neuroblastoma cell nuclei shown by confocal scanning laser microscopy. Nature 317,748-749. 

Shuman, H. (1987) Contrast in confocal scanning microscopy with a finite detector. J. Microscopy 149, 67-71. 

Figure 14.4. Confocal scanning-beam laser microscope. (From Ref. 6, with permission from the Electrochemical Society.)...

Wash array free of unbound antigens, then perform confocal scan. Analyze data. 

Fig. 4.7 Confocal scanning microscope image of (top) a spinodal phase separation structure Ti02 thin film, and (bottom) the cross-sectional height profile of the above picture across the solid line. Reproduced with permission from Ref. [100].

Pinto, M.C. and Macias, P. (1995) Determination of intraparticle immobilized enzyme distribution in porous support by confocal scanning microscopy. Biotechnology Techniques, 9(7), 481-486. 

Sherar, M. D., Noss, M. B., and Foster, F. S. (1987). Ultrasound backscatter microscopy images the internal structure of living tumour spheroids. Nature 330,493-5. [174] Shimada, H. (1987). Propagation of multi-mode ultrasonic pulses in non-destructive material evaluation. In Ultrasonic spectroscopy and its application to Materials science (ed. Y. Wada), pp. 50-6. Ministry of Education, Science and Culture, Japan. [148] Shotton, D. M. (1989). Confocal scanning optical microscopy and its applications for biological specimens. J. Cell. Sci. 94,175-206. [177,200]... 

The APMS used for this separation had an average particle size of 4-10 pm Normal phase HPLC of ferrocene and acetylferrocene performed with non-porous 1-3 pm spheres prepared in basic solution showed only one broad peak with no separation of the target molecules. Similarly, 20 pm spheres prepared in acidic solution showed no resolution of the ferrocenes (Figure 1). This indicates that particle size has some effect on the quality of the HPLC separation, but surface area is the major factor provided that the molecules to be separated can access the interiors of the mesoporous particles, which is dependent upon the pore size. (Experiments performed on APMS using confocal scanning laser microscopy indicated that these particles are porous throughout their interiors). 

Confocal scanning microscopy 129 Conformations of molecules 43 - 45 anti 43... 

Shotton, D. M. (1989) Confocal scanning optical microscopy and its applications for biological specimens J Cell Sci 94, 175-206... 

Blonk, J.C.G. and Vanaalst, H. 1993. Confocal scanning light-microscopy in food research. Food Res. Intematl. 26 297-311. 

Heertje, I., Vandervlist, P., Blonk, J.C.G., Hen-drickx, H., and Brakenhoff, G.J. 1987. Confocal scanning laser microscopy in food-research— Some observations. Food Microstructure 6 115-120. 

Heertje, 1., Nederlof, J., Hendrickx, H., and Lucas-senreynders, E.H. 1990. The observation of the displacement of emulsifiers by confocal scanning laser microscopy. Food Structure 9 305-316. 

S. Inoue, Foundations of Confocal Scanned Imaging in Light Microscopy. In J. 

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