Fluorescence microscopy stains

Biggs A R 1985 Detection of impervious tissue in tree bark with selective histochemistry and fluorescence microscopy. Stain Tech 60 299-304... 

It had been found that if bacteria are stained with acridine orange and examined under fluorescent microscopy, viable, as dishnct from dead, cells fluoresce with an orange-led hue. This basic observation has been adapted to an ingenious method of determining bacterial content and may be completed within 1 hour. 

Tlie morphology of some bacteria, especially those that form spores, is distinctive enough under the light microscope to have value for identification. This means that differential staining techniques, such as the Gram stain or acid-fast stain, and fluorescence microscopy may help to determine the iden-... 

During the past two decades, cell-biological and biomedical research has greatly benefited from innovations in fluorescence microscopy. Both the increase in the repertoire of fluorescence staining techniques at the (sub)cellular level and the development of a multitude of novel fluorescence microscopy techniques contributed significantly. 

DNA molecules on gel are stained with ethidium bromide and observed using epi-fluorescent microscopy. 

Fig. 26 MR images of tumors of mice after they were injected with (a) paramagnetic av[33-specific RGD-liposomes and (b) nonspecific paramagnetic RAD-liposomes. (c, d) Fluorescence microscopy of 10 pm sections from dissected tumors revealed a distinct difference between tumors of mice that were injected with RGD-liposomes (c) or RAD-liposomes (d). Vessel staining was done with an endothelial cell-specific FITC-CD31 antibody. The red fluorescence represents the liposomes and the green fluorescence represents blood vessels. RGD-liposomes were exclusively found within the vessel lumen or associated with vessel endothelial cells (c), whereas RAD-liposomes (d) were also found outside blood vessels within the tumor (Adapted from [88])...

Fluorescence Microscopy of Aniline Blue Stained Pistils... 

Purposes of the Application of Fluorescence Microscopy to Pistils Stained by Aniline Blue... 

Plant Cells and Tissues Structure-Function Relationships. Methods for the Cytochemical/Histochemical Localization of Plant Cell/Tissue Chemicals. Methods in Light Microscope Radioautography. Some Fluorescence Microscopical Methods for Use with Algal, Fungal, and Plant Cells. Fluorescence Microscopy of Aniline Blue Stained Pistils. A Short Introduction to Immunocytochemistry and a Protocol for Immunovi-sualization of Proteins with Alkaline Phosphatase. The Fixation of Chemical Forms on Nitrocellulose Membranes. Dark-Field Microscopy and Its Application to Pollen Tube Culture. Computer-Assisted Microphotometry. Isolation and Characterization of... 

The stained nuclei stand out in vivid contrast to other fluorescently labeled cell structures when observed by fluorescence microscopy. 

Fig. 9.7 Indirect immunofluorescent staining of SWCNTs-treated F1EK293 cells for day 1-5 by fluorescent microscopy (x 630). Green cadherin red collagen blue cellular nucleus staining with DAPI. The expression levels of cadherin and collagen IV in cells decreased gradually as the culture days increased F1EK293 cells gradually detached from the cell populations as the culture days increased (Grunlan et al., 2004. With permission from Elsevier) (See Color Plates)...

The immunocytochemical staining of cytochrome C offers another alternative since, upon exposure to apoptotic stimuli, cytochrome C is rapidly released into the cytosol, an event that may be required for the completion of apoptosis in some systems (L2). The effect of cytosolic cytochrome C is thought to be the activation of caspases. The immunocytochemical staining of cytochrome C localized in mitochondria in healthy cells or diffused in the cell cytoplasm with monoclonal antibody (Promega) after induction of apoptosis, as detected by fluorescence microscopy, can be used for monitoring apoptosis (L2, M7, S8, T6). It is also a simple, rapid, specific mefhod for quanfifafive assessment of apoptotic cells. 

Fig-3 Fluorescence microscopy of Blastocystis revealing MitoTracker Green uptake of MLOs with DAPI colocalization. The MLOs are situated very close to the nucleus (large DAPI-stained structures) and are clustered at opposite poles of the cell... 

Successful cell surface display of the protein can be verified by inducing gene expression via addition of anhydrotetracycline to the culture medium and immunofluorescence staining of the cells using an antibody directed against the protein to be displayed (Protocol 1). Analysis can be performed by fluorescence microscopy or flow cytometry. 

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