Immunocytochemical staining

Abendroth CS, Dabbs DJ. Immunocytochemical staining of unstained versus previously stained cytologic preparations. Acta Cytol. 1995 39 379-386. 

Evers P, Uylings HB. Effects of microwave pretreatment on immunocytochemical staining of vibratome sections and tissue blocks of human cerebral cortex stored in formaldehyde fixative for long periods. /. Neurosci. Methods 1994 55 163-172. 

NSC content was assessed in a population of cells expanded on EGF-His-immobilized surfaces for 5 days. Cells were immunocytochemically stained with antibodies specific for nestin, a marker for NSCs, and (1-tubulin III (pill),... 

Immunocytochemical staining with antibody-gold probes is a powerful way to detect, localize, and quantify antigen molecules in tissue sections and cells (Figure 24.3). Metabolic processes can be followed, epitope mapping of the structural characteristics of macromolecules can be... 

In tissue culture, astrocytes, hippocampal neurons and arterial myocytes all express al diffusely in their plas-malemma. In contrast, immunocytochemically stained a2 in astroglia and a3 in myocytes and neurons display reticular patterns and colocalize with the Na+/Ca2+ exchanger (NCX) (Fig. 5-6). These plasmalemma reticular patterns coincide with the patterns of staining for junctional complexes on sarcoplasmic reticulum and, in astroglia and neurons, junctional complexes on endoplasmic reticulum [23]. 

Roth J, Bendayan M, Carlemalm E. Villager W, Garavito M. Enhancement of structural preservation and immunocytochemical staining in low temperature. JHisto-chem Cytochem 1981 29 663-671. 

Immunocytochemical staining methods, for flax fiber, 11 599 Immunodiffusion, 14 136 Immunoelectrophoresis, 14 136... 

Janssens, P., Komaat, N., Tieleman, R., Monnens, L., and Willems, J. (1992) Localizing the site of hematuria by immunocytochemical staining of erythrocytes in urine. Clin. Chem. 38, 216-222. 

Berryman, M. A. and Rodewald, R. D. (1990) An enhanced method for postembedding immunocytochemical staining which preserves cell membranes. J. Histochem. Cytochem. 38,159-170. 

Cell signals Cytoplasmic-nuclear translocation (e.g., proportioning of immunocytochemical stain for NFKB with inflammation and AP-1 and NRF2 with oxidative stress)... 

The immunocytochemical staining of cytochrome C offers another alternative since, upon exposure to apoptotic stimuli, cytochrome C is rapidly released into the cytosol, an event that may be required for the completion of apoptosis in some systems (L2). The effect of cytosolic cytochrome C is thought to be the activation of caspases. The immunocytochemical staining of cytochrome C localized in mitochondria in healthy cells or diffused in the cell cytoplasm with monoclonal antibody (Promega) after induction of apoptosis, as detected by fluorescence microscopy, can be used for monitoring apoptosis (L2, M7, S8, T6). It is also a simple, rapid, specific mefhod for quanfifafive assessment of apoptotic cells. 

Perrot-Applanat, M., Groyer-Picard, M.-T., Vu Hai, M. T., Pallud, C., Spyratos, F., and Milgrom, E. 1989. Immunocytochemical staining of progesterone receptor in paraffin sections of human breast cancers. Am. J. Pathol. 735 457-468. 

Schultz, D. S., Katz, R. L., Patel, S., Johnston, D., and Ordonez, N. G. 1992. Comparison of visual and CAS-200 quantitation of immunocytochemical staining in breast carcinoma samples. Anal. Quant. Cytol. Histol. 4 35-40. 

Shimada, A., Kimura, S., Abe, K, Nagasaki, K., Adachi, I.,Yamaguchi, K., Susuki, M. F., Nakajima, T., and Miller, L. S. 1985. Immunocytochemical staining of estrogen receptor in paraffin sections of human breast cancer by use of monoclonal antibody Comparison with that in frozen sections. Proc. Natl. Acad. Sci. USA 32 4803-1807. 

Leong AS-Y, Parkinson R, Milios J. Thick cell membranes revealed by immunocytochemical staining. A clue to the diagnosis of malignant mesothelioma. 

Fixatives for prefixation of tissue prior to immunocytochemical staining The best fixative for the preservation of cellular mor-... 

Fig. 36. Immunocytochemical staining patterns of two monoclonal anti-bodies directed against non-phosphorylated and phosphorylated neurofilaments were studied in the cerebellum of developing normal rats. A. Non-phosphorylated neurofilaments on postnatal day 11. B. Day 21. Basket cell axons form a characteristic brush-like plexus around the initial segment of the Purkinje cell axon. C. Phosphorylated neurofilaments on postnatal day 13, D. Postnatal day 21. Stained filaments are restricted to Purkinje cell and basket cell axons and are absent from the Purkinje cell cytoplasm. Calibration bars in A and C 30/tm, in B and D 10/tm. Marc et al. (1986).

Fig. 7. GABAergic neurons in the MOB. Immunocytochemical staining of GABAergic cells (A) or GAD-containing terminals (B) in MOB. Bar in B, 100 jim.

Swaab DF, Pool CW, VanLeenwen FW. Can specificity evet be proven in immunocytochemical staining J Histochem Cytochem. 1977 25 388. 

Halliday BE, Slagel DD, Elsheikh TE, et al. Diagnostic utility of MIC-2 immunocytochemical staining in the differential diagnosis of small blue cell tumors. Diagn Cytopathol. 1998 19 410-416. 

Lidang Jensen M, Johansen P. Immunocytochemical staining of serous effusions an additional method in the routine cytology practice Cytopathology. 1994 5 93-103. 

Tomita T. Immunocytochemical staining patterns for parathyroid hormone and chromogranin in parathyroid hyperplasia, adenoma and carcinoma. Endocr Pathol. 1999 10 145-156. 

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