Saccharomyces cerevisiae fermentation

Continuous extraction loop reactor Ethanol production with glucose fermentation Saccharomyces cerevisiae 126... 

Index Entries Bioethanol corn fiber oil Escherichia coli pentose fermentations Saccharomyces cerevisiae p-glucosidase. 

Kuyper, M., Toirkens, M. J., Diderich, J. A., Winkler, A. A., Van Dijken, J. R, Pronk, J. T. (2005). Evolutionary Engineering of mixed-sugar utilization by a xylose-fermenting Saccharomyces cerevisiae strain. FEMS Yeast Research, 5, 925-934. 

Bettiga, M., Hahn-Hagerdal, B., Gorwa-Grauslund, M. F. Comparing the xylose reductase/ xylitol dehydrogenase and xylose isomerase pathways in arabinose and xylose fermenting Saccharomyces cerevisiae strains. Biotechnol Biofuels. 2008, 1, 16. 

Alcoholic Fermentation. Certain types of starchy biomass such as com and high sugar crops are readily converted to ethanol under anaerobic fermentation conditions ia the presence of specific yeasts Saccharomyces cerevisia and other organisms (Fig. 6). However, alcohoHc fermentation of other types of biomass, such as wood and municipal wastes that contain high concentrations of cellulose, can be performed ia high yield only after the ceUulosics are converted to sugar concentrates by acid- or enzyme-catalyzed hydrolysis ... 

Alternative Step D Reduction with a Reductate — Sucrose (1 kg) is dissolved in water (9 liters) in a 20-liter bottle equipped with a gas trap. Baker s yeast Saccharomyces cerevisiae, 1 kg) is made into a paste with water (1 liter) and added to the sucrose solution with stirring. After lively evolution of gas begins (within 1 to 3 hours), 3-morpholino-4-(3-tert-butylamino-2-oxopropoxy)-1,2,5-thiadiazole hydrogen maleate [1.35 mols, prepared by reaction of the 3-morpholino-4-(3-tert-butylamino-2-oxopropoxy)-1,2,5-thiadiazole with an equimolar quantity of maleic acid in tetrahydrofuran]. The mixture is allowed to stand until fermentation subsides, after which the bottle is kept in a 32°C incubator until all fermentation has ended (in approximately 1 to 3 days). The yeast is filtered off with addition of diatomaceous earth and the filtrate is evaporated to dryness to give S-3-mor-pholino-4/3-tert-butylamino-2-hydroxypropoxy)-1,2,5-thiadiazole, MP 195° to 198°C (as hydrogen maleate), according to U.S. Patent 3,619,370. 

For preparative purposes fermenting baker s yeast (Saccharomyces cerevisiae) is commonly used instead of a purified enzyme preparation. However, isolated pyruvate decarboxylates can also be used30. In this context, the most important substrate is benzaldehyde31 which is converted by n-glucosc fermenting yeast to (7 )-l-hydroxy-l-phenyl-2-propanone. This conversion has gained considerable industrial importance because ( )-l-hydroxy-1-phenyl-2-propanonc is an important precursor for the synthesis of (-)-cphedrin. 

CASE STUDY ETHANOL FERMENTATION IN AN IMMOBILISED CELL REACTOR USING SACCHAROMYCES CEREVISIAE... 

Fermentation of sugar by Saccharomyces cerevisiae, for production of ethanol in an immobilised cell reactor (ICR), was successfully carried out to improve the performance of the... 

Keywords immobilised cell reactor (ICR) Saccharomyces cerevisiae ethanol fermentation encapsulated beads calcium alginate... 

Dining batch fermentation of Saccharomyces cerevisiae, other influential parameters can adversely influence the specific rate of growth, and inhibition can be caused either by... 

Najafpour, G.D., Younesi, H. and Ku Ismail, K.S., Ethanol Fermentation in Immobilized Cell Reactor (ICR) Using Saccharomyces cerevisiae , Bioresource Technology, vol. 92/3, 2004, pp. 251-260. 

In the reduction of racemic /i-ketosulphoxides (e.g. 464a) with actively fermenting yeast (Saccharomyces cerevisiae) the enantiomers are reduced at sufficiently different rates to allow isolation of optically active /1-hydroxy sulphoxide 524 and unreacted optically active /1-ketosulphoxide with at least 95% optical purity617,618 (equation 323). 

Preparation by fermentation of Saccharomyces cerevisiae (baker yeast) with addition of L- or DL-methionine, lyse of cells with ethyl acetate and purification by ion-exchange chromatography. 

Example 12.7 Develop a model for the anaerobic batch fermentation of glucose to ethanol and coproduct CO2 using Saccharomyces cerevisiae. The starting mixture contains 10% glucose. The inoculum is 0.0005 w/w. Product inhibition stops cell growth at 14% ethanol. Assume ka = 0 but include the cannibalization of cellular material beginning when the substrate is completely consumed. 

FIGURE 12.4 Simulation of a batch Saccharomyces cerevisiae fermentation. 

A literature value for the Monod constant for a Saccharomyces cerevisiae fermentation is. S ,s = 25mg/liter. How does this affect the simulation in Example 12.7 ... 

In current industrial practice, benzaldehyde is added to fermenting baker s yeast Saccharomyces cerevisiae) with resultant PAC production occurring from the yeast-derived pyruvate. Typically PAC concentrations of 12-15 g F are produced at yields of 65-70% theoretical in a 10-12 h biotransformation process. [2], Appreciable concentrations of benzyl alcohol are produced as by-product due to oxidoreductase activity in the fermentative yeast. 

Drdak, M. et al., Red beet pigment composition effects of fermentation by different strains of Saccharomyces cerevisiae, J. Food Sci., 57, 935, 1992. 

The catalytic capacity of several excreting pectolytic enzymes obtained from various yeast strains was examined using in vivo and biochemical techniques. Of the 33 yeast strains studied 30 were isolated from champagne wine during alcoholic fermentation. Only one yeast strain was found to excrete pectolytic enzymes and was identified as Saccharomyces cerevisiae designated SCPP. Three types of pectolytic enzymes were found to be excreted by SCPP polygalacturonase (PG), pectin-lyase (PL) and pectin-esterase (PE) [1]. 

Phenylacetylcarbinol 15 Ephedrine Treatment for asthma Lyase Saccharomyces cerevisiae Single-stage fermentation [9]... 

Kuhn, K. M., DeRisi, J. L., Brown, P. O., and Samow, P. (2001). Global and specific translational regulation in the genomic response of Saccharomyces cerevisiae to a rapid transfer from a fermentable to a nonfermentable carbon source. Mol. Cell Biol. 21, 916-927. 

---