Ever tissue

OHnga, R, Meijer, D.K.F., Slooff, M.J.H., and Groothuis, G.M.M. (1997). Liver slices in in vitro pharmacotoxicology with special reference to the use of human Ever tissue. Toxicol. In Vitro 12 11. 

Evers BM (2006) Neurotensin and growth of normal and neoplastic tissues. Peptides 27 2424—2433... 

The high affinity of the decarboxylase enzyme for its substrate (10 pM in the brain) makes it unlikely that this stage could ever become rate-limiting for the pathway as a whole. Nevertheless, the for this enzyme is considerably higher than tissue concentrations of 5-hydroxytryptophan and so, again, supply of this substrate is likely to be a crucial factor. 

Geography and habitat variability affect MeHg prodnctiorr, bioaccessibility, and uptake into wildlife. Interpretation of mercttry in wildlife also reqtrires a working knowledge of sex, age, and tissue differences (Evers et al. 2005). Biogeochemical differences in aquatic and terrestrial systems are partictrlarly important determinants of Hg methylation, as discussed in previons chapters for water and fish. 

Evers P, Uylings HB. An optimal antigen retrieval method suitable for different antibodies on human brain tissue stored for several years in formaldehyde fixative. I. Neurosci. Methods 1997 72 197-207. 

Evers P, Uylings HB, Suurmeijer AJ. Antigen retrieval in formaldehyde-fixed human brain tissue. Methods 1998 15 133-140. 

Evers P, Uylings HB. Effects of microwave pretreatment on immunocytochemical staining of vibratome sections and tissue blocks of human cerebral cortex stored in formaldehyde fixative for long periods. /. Neurosci. Methods 1994 55 163-172. 

PGA was one of the very first degradable polymers ever investigated for biomedical use. PGA found favor as a degradable suture, and has been actively used since 1970 [45 -7]. Because PGA is poorly soluble in many common solvents, limited research has been conducted with PGA-based drug delivery devices. Instead, most recent research has focused on short-term tissue engineering scaffolds. PGA is often fabricated into a mesh network and has been used as a scaffold for bone [48-51], cartilage [52-54], tendon [55, 56], and tooth [57]. 

Since 1988, the methods that we use to isolate cDNAs of alkaloid biosynthesis have become ever more facile and sensitive, allowing for more efficient cDNA identification. We do not, however, yet understand enough about the cellular localization of alkaloid formation or about the nature of the catalysts to move completely away from enzymology and biochemistry and to use only molecular genetic techniques to dissect these biosynthetic pathways. Even our most recently successful cDNA isolations and identifications involved classical protein purification. We are beginning now to use proteomics and EST sequencing to identify natural product biosynthetic cDNAs, but these approaches are more feasible when a specialized cell/tissue type in which secondary metabolite biosynthetic pathways are active, can be isolated and used as a protein or RNA source. 

Even though my thinking is not as sharp and clear, my ability to reason and to communicate and understand is probably deeper and broader than ever. Who knows, I could have tissue damage in my brain from the swelling and numbness caused by chemicals, but I am doing more cognitive and intellectual work than I ever have in my life. It s a different kind of thinking. 

Albert H. Coons was the first to attach a fluorescent dye (fluorescein isocyanate) to an antibody and to use this antibody to localize its respective antigen in a tissue section. Fluorescein, one of the most popular fluorochromes ever designed, has enjoyed extensive application in immunofluorescence labeling. For many years, classical fluorescent probes such as FITC or Texas red (TR) have been successfully utilized in fluorescence microscopy. In recent decades, brighter and more stable fluorochromes have continually been developed (see Table 14.1). Marketed by a number of distributors, cyanine dyes, Cy2, Cy3, Cy5, Cy7, feature enhanced water solubility and photostability as well as a higher fluorescence emission intensity as compared to many of the traditional dyes, such as FITC or TR. 

It is obvious that one should not expect to find in the literature extensive information regarding the composition of the brains, livers, or even muscles of healthy human individualsespecially so since repeated samples would have to be taken for analysis in order to determine conclusively the importance of inter-individual differences. The best that could be hoped for would be extensive "horizontal" studies relative to the composition of blood, secretions, etc., of individual human specimens and, perhaps, more comprehensive data including tissue composition with respect to animals. However, satisfactory studies of this sort have seldom, if ever, been made. More often than not, such horizontal studies as have been made have not been published in complete enough form to give the kind of information needed to answer the questions which we are considering. 

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