Cold sterilization

Crosslinking improves resistance to heat, stress cracking and abrasion, and also reduces permanent set after loading, residual monomers and/or oligomers and VOCs (volatile organic compounds). Irradiation is a cold sterilization process. 

Setup a petri dish containing about 5-10 ml of cold, sterile PBS before removing the spleen from the mouse. 

Place the spleen in a sterile, glass petri dish containing a few ml of cold, sterile PBS. 

Add a few drops of cold, sterile PBS to the spleen on the wire mesh to keep it moist. 

Uses. As broad-spectrum antimicrobial cold sterilant/disinfectant for hospital equipment as tanning agent for leather as tissue fixative as cross-linking agent for proteins as preservative in cosmetics as therapeutic agent for warts, hyperhidrosis, and dermal mycotic infections in X ray processing solutions and film emulsion as a disinfectant in the beauty industry... 

Glutaraldehyde has caused an allergic contact dermatitis in hospital workers using it as a cold sterilant or in handling recently processed X ray film. It appears to be a strong sensitizer. In general, reactions present as a vesicular dermatitis of the hands and forearms. Rubber gloves do not appear to afford complete protection. In unsensitized individuals it acts as a mild skin irritant. 

Inspect the cells on d 3, they should be almost fully confluent on the flask base. Discard the medium, add 10 mL of cold sterile PBS containing 0.02% ethylene diamine tetraacetic acid and leave for a few minutes to allow the cells to detach. Split the cells equally between two flasks and add 75 mL of RPMI 1640 medium/5% FBS to each flask and return to the incubator. 

On d 4, remove the medium from each flask and replaced with 20 mL of cold sterile PBS containing 0.02% ethylene diamine tetraacetic acid. After a few minutes the cells will detach from the flask surface. Harvest the cells by gently tapping the flask and pouring the cell suspension in to sterile universal containers. Wash the cells by centrifugation at 300g and resuspend in 10 mL of cold PBS. 

Cold sterilization of beer using microfiltration was introduced on a commercial scale in 1963. The process was not generally accepted at that time, but has recently become more common. Sterilization of beer and wine is much less stringent than pharmaceutical sterilization. The main objective is to remove yeast cells, which are quite large, so the product is clear and bright. Bacterial removal is also desirable a 106 reduction in bacteria is equivalent to the best depth filters. The industry has found that 1-p.m filters can remove essentially all the yeast as well as provide a 106 reduction in the common bacteria found in beer and wine. Because the cost structure of beer and wine production is very different from that... 

Pipette 50 pL of electrocompetent HB101 cells (19), with an efficiency of transformation of at least 107 CFU/pg of DNA, into an ice-cold sterile microfuge tube and place it on ice. 

The care and development of a wine is by no means terminated when the wine is bottled. Once bottled, the development of the wine is evidenced first by the disappearance of bottle sickness followed, over a longer period of time, by the development of maturity and complexity of the wine within the bottle. Having the wine in the winery during its early stages of development also affords the winemaker the opportunity to be assured of the sterility of cold-sterile bottled wine. 

Microfiltration with inorganic membranes is a promising alternative. As early as 1964 porous silver membranes (composed of permanently molecular bonded pure silver particles) in the disk form were commercially available. Silver membranes with a maximum pore diameter of 1.2 pm were tested successfully on the pilot scale for cold sterilization of beer to remove any organisms that can cause spoilage in closed... 

The safety and biocidal efficacy of glutaral has led to its endorsement by the CDC and WHO as a substitute for formaldehyde in high-level disinfection and cold sterilization (1,2). Glutaral is used in a 2% aqueous solution buffered to a pH of about 8 for sterilization of endoscopic and dental equipment and for other equipment that cannot be sterilized by heat. 

The white pellet is washed with ice-cold sterile water by adding 2 ml of water and centrifuging as in Step 4. 

Harvest cells by centrifugation at 2000x g for 5 min at 4°C, and wash the cell pellets twice in ice-cold sterile distilled water. 

Ultrafiltration processes are used to recover or concentrate a particular species in the retentate, as is exemplified by latex concentration, or to obtain a purified permeate as in sewage treatment. Ultrafiltration is a means of cold sterilization for many heat-sensitive liquids whose sterilization could not be carried out earlier. Thus, in the food industry draft beer provides an interesting example of a product whose feasibility as a commercial beverage has been an outcome of ultrafiltration cold sterilization by ultrafiltration retains the esters on account of which the beer has its draft flavour. Similarly, ultrafiltration plays a key role in the pharmaceutical industry for the commercial-scale preparation of sterile water and antibiotics. 

Fungitex R. [Ciba-Geigy/Dyestuffs] Domiphen bromide antimicrobial, fim-gicide for use in mouthwashes, antiseptics, cold sterilization. 

Fruit Juice. Apple juice processors are using UF to increase juice recovery, improve clarity, reduce materials cost, and to cold sterilize for aseptic packaging. 

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