Escherichia coli cloning

Selected strains of the bacterium Pseudomonas putida were found to yield chiral sulfoxides Ph-S(0)-R (R or -configuration) without sulfone formation. Similar results were obtained with an Escherichia coli clone 1112],... 

S. Cohen, A. Chang, H. Boyer, R. Helling Published a paper in the Proceedings of the National Academy of Sciences showing that a recombinant DNA molecule can be maintained and replicated in Escherichia coli ( cloning )- Boyer received the 1997 Nobel Prize in Chemistry for uncovering the enzymatic mechanism for synthesis of ATP... 

Lai, V., Wang, L., Reeves, P.R. Escherichia coli clone Sonnei (Shigella sonnei) had a chromosomal O-antigen gene cluster prior to gaining its current plasmid-borne O-antigen genes. J Bacteriol 180 (1998) 2983-2986. 

Mouse caveolin-1, C-terminal fused to yellow fluorescent protein (YFP pEX EFl Cav-l-YFP ATCC). The plasmid is supplied in an Escherichia coli clone and can be propagated using standard bacterial propagation techniques with kanamycin as selection pressure. Purify the plasmid using a maxi-prep kit from Qiagen, Sigma or other vendors. 

Xu, H.H., L. Real, and M.W. Bailey. 2006. An array of Escherichia coli clones over-expressing essential proteins a new strategy of identifying cellular targets of potent antibacterial compounds. Biochem. Biophys. Res. Commun. 349, 1250-1257. 

Ashiuchi M, Soda K, Misono H. (1999). A poly-y-glutamate synthetic system of Bacillus subtilis IFO 3336 gene cloning and biochemical analysis of poly-y-glutamate produced by Escherichia coli clone cells. Biochem Biophys Res Commun, 263, 6-12. 

Devine, J. H., et al. (1993). Luciferase from the east European firefly Luciola mingrelica cloning and nucleotide sequence of the cDNA overexpression in Escherichia coli and purification of enzyme. Biochim. Bio-phys. Acta 1173 121-132. 

Tatsumi, H., Masuda, T., Kajiyama, N., and Nakano, E. (1989). Luciferase cDNA from Japanese firefly, Luciola cruciata cloning, structure and expression in Escherichia coli. J. Biolumin. Chemilumin. 3 75-78. 

A number of allergens from both honey bee and vespid venoms have been cloned and expressed by either Escherichia coli or baculovirus-infected insect cells (table 1) phospholipase Aj [20], hyaluronidase [21], acid phosphatase [13] and Api m6 [14] from honey bee venom, as well as antigen 5 [22], phospholipase A and hyaluronidase [23] from vespid venom, and dipeptidylpeptidases from both bee and Vespula venoms [15, 16]. Their reactivity with human-specific IgE antibodies to the respective allergens has been documented [11-16, 22, 23] and their specificity is superior... 

Shine J., Eettes I., Lan N.C.Y., Roberts J.L. Baxter J.D. (1980) Expression of cloned j 8-endorphin gene sequences hy Escherichia coli. Nature, 285,456-461. 

BUGOS R 0 and yamamoto h y (1996) Molecular cloning of violaxanthin de-epoxidase from romaine lettuce and expression in Escherichia coli, Proc Natl Acad Sci, 93, 6320-25. 

Recently, a potential cytosolic component of the MEP precursor pathway, xylulose kinase, has been cloned and tested for function in an Escherichia coli complementation system. " The kinase activates exogenous xylulose in the cytoplasm. DXP is the precursor for DXS, which resides in the plastid, suggesting the activated substrate must be transported into the plastid. Another xylulose kinase homologue in Arabidopsis that contains a plastid targeting sequence was not active in the E. coli system, suggesting that it may have some other function in the plastid. Perhaps plant and bacterial tissue cultures may be fed xylulose to condition accumulation of isoprenoid metabolites. 

Sprenger, G.A. et al.. Identification of a thiamin-dependent synthase in Escherichia coli required for the formation of the 1-deoxy-D-xylulose 5-phosphate precursor to isoprenoids, thiamin, and pyridoxol, Proc. Natl. Acad Sci. USA 94, 12857, 1997. Lange, B.M. et al., A family of transketolases that directs isoprenoid biosynthesis via a mevalonate-independent pathway, Proc. Natl. Acad Sci. USA 95, 2100, 1998. Lois, L.M. et al., Cloning and characterization of a gene from Escherichia coli encoding a transketolase-like enzyme that catalyzes the synthesis of D-1- deoxyxylulose 5-phosphate, a common precursor for isoprenoid, thiamin, and pyridoxol biosynthesis, Proc. Natl. Acad. Sci. USA 95, 2105, 1998. 

Cunningham, F.X. Jr. et ah. Cloning and functional expression in Escherichia coli of a cyanobacterial gene for lycopene cyclase, the enzyme that catalyzes the biosynthesis of beta-carotene, FEBS Lett. 328, 130, 1993. 

Wu J-F, C-W Sun, C-Y Jiang, Z-P Liu, S-J Liu (2005) A novel 2-aminophenol 1,6-dioxygenase involved in the degradation of p-chloronitrobenzene by Comamonas strain CNB-1 purification, properties, genetic cloning and expression in Escherichia coli. Arch Microbiol 183 1-8. 

Miura K, Y Tomioka, H Suzuki, M Yonezawa, T Hishinuma, M Mizugaki (1997) Molecular cloning of the nemA gene encoding A -ethylmaleimide reductase from Escherichia coli. Biol Pharm Bull 20 110-112. 

Mondello FJ (1989) Cloning and expression in Escherichia coli of Pseudomonas strain LB400 genes encoding polychlorinated biphenyl degradation. J Bacteriol 171 1725-1732. 

Stalker DM, KE McBride (1987) Cloning and expresssion in Escherichia coli of a Klebsiella ozaenae plasmid-borne gene encoding a nitrilase specific for the herbicide Bromoxynil. J Bacterial 169 ... 

Zylstra GJ, LP Wackett, DT Gibson (1989) Trichloroethylene degradation by Escherichia coli containing the cloned Pseudomonas putida FI toluene dioxygenase genes. Appl Environ Microbiol 55 3162-3166. 

Ratnatilleke A, JW Vrijbloed, JA Robinson (1999) Cloning and sequencing of the coenzyme B,2-binding domain of isobutyryl-CoA mutase from Streptomyces cinnamonensis. Reconstitution of mutase activity and characterization of the recombinant enzyme produced in Escherichia coli. J Biol Chem 274 31679-31685. 

Neumann A, G Wohlfarth, G Diekert (1998) Tetrachloroethene dehalogenase from Dehalospirillum multivorans. cloning, sequencing of the encoding genes, and expression of the pceA gene in Escherichia coli. J Bacterial 180 4140-4145. 

Chen C-M, Q-Z Zhuang, Z Zhu, BL Wanner, CT Walsh (1990) Molecuar biology of carbon-phosphorus bond cleavage Cloning and sequencing of the phn (psiD) genes involved in alkylphosphonate uptake and C-P lyase activity in Escherichia coli. J Biol Chem 265 4461-4471. 

Fig. 1 Schematic outline of procedures employed in the synthesis of a cDNA gene copy from a polyadenylated mRNA template, insertion of the cDNA into a bacterial plasmid vector by a homopolymer tailing strategy, and cloning of the recombinant plasmid in an Escherichia coli host.

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