Luciola cruciata

Fig. 1.1 The firefly Luciola cruciata (male) drawn by Sakyo Kanda (1874-1939), a pioneer of the study of bioluminesccnce in Japan, showing his extraordinary artistic talent (reproduced from Kanda, 1935).

Kishi, Y., et al. (1968). Luciferin and luciopterin isolated from the Japanese firefly, Luciola cruciata. Tetrahedron Lett., pp. 2847-2850. 

Ohmiya, Y., Ohba, N., Toh, H., and Tsuji, F. I. (1994). Comparative aspects of the bioluminescence reactions of Hotaria parvula, Luciola cruciata, and Pbotinus pyralis. In Campbell, A. K., etal. (eds.), Biolumin. Chemi-lumin. Proc. Int. Symp., 8th 1994, pp. 572-575. Wiley, Chichester, UK. 

Tatsumi, H., Masuda, T., Kajiyama, N., and Nakano, E. (1989). Luciferase cDNA from Japanese firefly, Luciola cruciata cloning, structure and expression in Escherichia coli. J. Biolumin. Chemilumin. 3 75-78. 

Beside that of Photinus pyralis, other luciferases have been described in the literature such as those from Luciola cruciata, Luciola lateralis [122], and Luciola mingrelica. Among them luciferase from the latter one has been employed for analytical purposes [123],... 

Luciola cruciata specimens were collected from 62 sites in Japan covering almost all the insect s distribution areas. Luciola lateralis from 46 sites in Japan and two sites in Korea. Luciola owadai fi om Kume-jima Island, the Okinawa Islands, Japan. 

Figure 1. Distribution pattern of CO II haplotype-groups in Luciola cruciata.

Ohba N. Synchronous flashing in the Japanese firefly, Luciola cruciata (Coleoptera Lampyridae). Sci Rept Yokosuka City Mus 1984 32 23-32. 

PPDK from Microbispora rosea subsp. Aerata (EC2.7.9.1) and thermostable Luciola cruciata firefly luciferase (EC 1.13.12.7) were obtained from Kikkoman Co. (Chiba, Japan). Pyrophosphate, Lysozyme and Proteinase K were purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). dNTP Mixture, Taq DNA Polymerase and lOx PCR buffer were acquired from Takara Shuzo Co., Ltd (Osaka, Japan). Primers were synthesized by Takara Shuzo Co., Ltd (Osaka, Japan). Luciferin was obtained from Sigma Chemical Co (St. Louis, MO). Perfect match (PCR Enhancer) was manufactured by Toyobo. Other reagents were of analytical grade. 

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