O-antigen gene clusters

Keywords O antigen Gram-negative bacteria O antigen diversity Structure O antigen gene cluster... 

Coimbra et al. proposed a comprehensive approach for molecular serotype determination by using long-range PCR to amplify E. coli O antigen gene clusters, followed by enzymatic restriction (PCR-RFLP). They used Mboll on 148 serogroups and the method proved to be effective for rapid characterization of the known E. coli O serotypes and for the detection of new O serotypes (Coimbra et al., 2000). The... 

More than 230 O antigen gene clusters have been reported, the majority being from E. coli, S. enterica and Shigella spp. For all of them the GC content of the cluster... 

In S. sonnei, which is different from the other Shigella strains in having a major deletion in the O antigen gene cluster between galF and gnd, the functional... 

E. sakazakii is an opportunistic pathogen that has been associated with food-borne illness in neonates. Only two major serotypes - Ol and 02 have been identified. The two O antigen gene clusters are located between the galF and gnd genes, and both have the Wzx/Wzy pathway (Mullane et al., 2008). 

Four V. cholerae O antigen gene clusters (Ol, 0139, 037 and 022) have been reported, all located between the gmhD and rjg genes, and all have the ABC transporter pathway (Chatterjee and Chaudhuri, 2004). 

The O antigen gene cluster of 022 is 35.9-kb long and closely related to that of... 

Sequence analyses of the Y. pseudotuberculosis O antigen gene clusters between hemH and gsk revealed a pattern of modules for different backbone structures and for different sidebranch sugars, with indications of homologous recombination events during evolution of the diversity (Skurnik et al., 2000 Pacinelli et al., 2002 ... 

Role of Plasmids in Evolution of O Antigen gene Clusters... 

Bogdanovich, T., Camiel, E., Fukushima, H., Skumik, M. Use of O-antigen gene cluster-specific PCRs for the identification and O-genotyping of Yersinia pseudotuberculosis and Yersinia pestis. J Clin Microbiol 41 (2003) 5103-5112. 

Coimbra, R.S., Grimont, F., Lenormand, P., Burguiere, P., Beutin, L., Grimont, P.A. Identification of Escherichia coli O-serogroups by restriction of the amplified O-antigen gene cluster (rfb-RFLP). Res Microbiol 151 (2000) 639-654. 

Cunneen, M.M., Reeves, P.R. The Yersinia kristensenii Oil O-antigen gene cluster was acquired by lateral gene transfer and incorporated at a novel chromosomal locus. Mol Biol Evol 24 (2007) 1355-1365. 

Feng, L., Han, W., Wang, Q., Bastin, D.A., Wang, L. Characterization of Escherichia coli 086 O-antigen gene cluster and identification of 086-specific genes. Vet Microbiol 106 (2005) 241-248. 

Feng, L., Wang, W., Tao, J., Guo, H., Krause, G., Beutin, L., Wang, L. Identification of Escherichia coli 0114 O-antigen gene cluster and development of an 0114 serogroup-specific PCR assay. J Clin Microbiol 42 (2004) 3799-3804. 

Lai, V., Wang, L., Reeves, P.R. Escherichia coli clone Sonnei (Shigella sonnei) had a chromosomal O-antigen gene cluster prior to gaining its current plasmid-borne O-antigen genes. J Bacteriol 180 (1998) 2983-2986. 

Samuel, G., Hogbin, J.P., Wang, L., Reeves, P.R. Relationships of the Escherichia coli 0157, Olll, and 055 O-antigen gene clusters with those of Salmonella enterica and Citrobacter freundii, which express identical O antigens. J Bacteriol 186 (2004) 6536-6543. 

Skurnik, M., Peippo, A., Ervela, E. Characterization of the O-antigen gene clusters of Yersinia pseudotuberculosis and the cryptic O-antigen gene cluster of Yersinia pestis shows that the plague bacillus is most closely related to and has evolved from Y. pseudotuberculosis serotype O lb. Mol Microbiol 37 (2000) 316-330. 

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