Enzyme profile

Shimazaki, Y., Sugarawa, Y, Manabe, T. Nondenaturing two-dimensional electrophoresis enzyme profile involving activity and sequence structure of cytosol proteins from mouse liver. Proteomics 2004, 4, 1406-1411. 

The adverse reaction enzymes profile from M DS Pharma including 41 enzymatic assays to predict moderate to serious adverse effects. 

The discussion to this point has focused on the isolation of intact mitochondria. By various chemical and physical treatments, mitochondria may be separated into their four components. This allows biochemists to study the biological functions of each component. For example, by measuring enzyme activities in each fraction, one can assign the presence of a particular enzyme to a specific region of the mitochondria. Studies of mitochondrial subfractions have resulted in a distribution analysis of enzyme activities in the four locations (Table E10.1). This type of study is often referred to as an enzyme profile or enzyme activity pattern and the enzyme may be considered a marker enzyme. For example, cytochrome oxidase, which is involved in electron transport, is a marker enzyme for the inner membrane. 

Comparison of Enzyme Profiles in Subgenera Within Genus Penicillium... 

Kohlhagen, S., Behm, C. A. Bryant, C. (1985). Strain variation in Hymenolepis diminuta enzyme profiles. International Journal for Parasitology, 15 479-83. 

Kurup VP, Resnick A, Scribner GH, Gunasekaran M, Fink JN Enzyme profile and immunochemical characterization of A. fumigatus antigens. J Allergy Clin Immunol 1986 78 1166-1173. Kothary MH, Chase TJ, Macmillan JD Correlation of elastase production by some strains of A. fumigatus with ability to cause pulmonary invasive aspergillosis in mice. Infect Immun 1984 43 320-325. 

The less distorted the enzyme pattern within the serum, the more profound is the underlying necrotic hepatocellular damage, i. e. the enzyme profile within the serum corresponds to that within the hepatocytes LDL > GOT > GPT > GDH. 

MacNamara, E., Goldberg, D.M. Serum enzymes and enzyme profiles in the diagnosis of liver and biliary tract disease. Surv. Dig. Dis. 1985 3 165-186... 

HepaRG Most major phase I and II enzymes Medium to high Enzyme profile not thoroughly characterized a few enzymes expressed only at a low level... 

It is known that all the organs and tissues display drug metabolism, which vary vastly qualitatively, quantitatively, and regionally [26, 27]. This complexity makes it even more important to characterize isolated cells or cell lines of the organs under study with respect to metabolic enzyme profile and activity. A series of questions should be posed, such as the following What should be known about a cell line before its use for experiments How to link the purpose of the study with appropriate cells or tools Is a single cell line appropriate or should tissue explant be used ... 

R6. Romanul, F. C. A., and Van der Meulen, J. P., Reversal of the enzyme profile of muscle fibres in fast and slow muscles by cross-innervation. Nature (London) 212, 1369-1370 (1966). 

Seven of the nineteen patients were treated with NAC and each had an elevated or equivocal plasma Bj concentration upon admission within 6 hr of overdose. The plasma B, levels continued to increase despite NAC administration in each patient but 13 hr after the overdose peak plasma B, concentrations had been reached, with levels of up to 20 times the upper reference limit being found. Thereafter, B, concentrations fell until, more than 24 hr after taking the overdose, normal B, concentrations were found in all but two patients. In contrast, to Bj, ALT activates were normal in all patients upon admission and never rose to values in excess of four times the upper reference limit. Examples of the enzyme profiles obtained in these patients are shown in Fig. 17. 

Myocardial infarction occurs when the blood supply to the heart muscle is blocked for an extended time. If this lack of blood supply, called ischemia, is prolonged, the myocardium suffers irreversible cell damage and muscle death, or infarction. When this happens, the concentration of cardiac enzymes in the blood rises dramatically as the dead cells release their contents into the bloodstream. Although many enzymes are liberated, three are of prime importance. These three enzymes, creatine phosphokinase (CPK), lactate dehydrogenase (LDH), and aspartate aminotransferase/serum glutamate-oxaloacetate transaminase (AST/SGOT), show a characteristic sequential rise in blood serum level following myocardial infarction and then return to normal. This enzyme profile, shown in the ac-... 

AST release is not specific to Ml, but is also found in many acute pathologies affecting the liver and skeletal muscle. The enzyme alanine aminotransferase (ALT) is sometimes measured as pan of the cardiac enzyme profile of tests (Fig. 3). ALT is found in high concentration in liver rather than muscle, and a normal ALT in the face of a raised AST confirms that liver pathology is not contributing to the raised enzyme levels. Appro.ximately 25% of patients with pulmonary embolus, which features in the differential diagnosis of Ml and which may or may not cause chest pain, show a raised AST concentration, aiul most also show an elevated LDH. 

Kp is a partition coefficient of the enzyme in agarose and kg is a mass transfer coefficient. Although predictions can be made about the bound-enzyme profile, it is not possible to directly verify them experimentally because of the protein impurities in heparinase. However, it is possible to measure the bulk heparinase concentration during the course of an immobilization and compare it to the model predictions. A series of immobilizations were conducted using intact agarose spheres with different cyanate ester concentrations and the bulk heparinase concentration monitored. 

The bound-enzyme profile under the normal immobilization conditions is given in Fig. 8 as a function of time and radial position. At each time, the profile was essentially uniform. At 4 h, which corresponded to the... 

Table 1 Comparison of H. Jecorina enzyme profile after 8 days growth on untreated or 230°C PBHW-pretreated Tifton 85 bermudagrass as substrate with that of commercial preparations. 

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