Enantiomeric determination

The study of biochemical natural products has also been aided through the application of two-dimensional GC. In many studies, it has been observed that volatile organic compounds from plants (for example, in fruits) show species-specific distributions in chiral abundances. Observations have shown that related species produce similar compounds, but at differing ratios, and the study of such distributions yields information on speciation and plant genetics. In particular, the determination of hydroxyl fatty acid adducts produced from bacterial processes has been a successful application. In the reported applications, enantiomeric determination of polyhydroxyl alkanoic acids extracted from intracellular regions has been enabled (45). 

Cass, Q.B., Lima, V.V., Oliveira, R.V., Cassiano, N.M., Degani, A.L.G., Pedrazzoli, P., Jr. (2003). Enantiomeric determination of the plasma levels of omeprazole by direct plasma injection using high-performance liquid chromatography with achiral-chiral columnswitching. J. Chromatogr. B 798, 275-281. 

Ichihara, H., Fukushima, T., Imai, K. (1999).. Enantiomeric determination of d- and L-lactate in rat serum using high-performance liquid chromatography with a cellulose-type chiral stationary phase and fluorescence detection. Anal. Biochem. 269, 379-385. 

Suckow, R.F., Zhang, M.F., Cooper, T.B. (1997). Enantiomeric determination of the phenyl-morphohnol metabolite of bupropion in human plasma using coupled achiral-chiral liquid chromatography. Biomed. Chromatogr. 11, 174-179. 

Enantiomeric determinations are typically done according to a normalized peak area (area%) calculation procedure. A reporting threshold solution containing the lowest level to be... 

Berzas Nevado, J. J., Guiberteau Cabanillas, C., Villasenor Llerena, M. J., and Rodriguez Robledo, V. (2005). Enantiomeric determination, validation and robustness studies of racemic citalopram in pharmaceutical formulations by capillary electrophoresis. /. Chromatogr. A 1072(2), 249—257. 

Enantiomeric Determination in Bulk Material and Pharmaceutical Formulations... 

Wainer, I. and Doyle, T., The direct enantiomeric determination of (-) and (+)-propranolol in human serum by high-performance liquid chromatography on a chiral stationary phase, J. Chromatogr., 306, 405, 1984. 

W.A. Tao et al., Rapid enantiomeric determination of a-hydroxy acids by electrospray ionization tandem mass spectrometry. Chem. Commun. 20, 2023-2024 (2000)... 

W.A. Tao et al., Quotient ratio method for quantitative enantiomeric determination by mass spectrometry. Anal. Chem. 74, 3783-3789 (2002)... 

L. Wu et al., Kinetic method for simultaneous enantiomeric determination of amino acid mixtures. J. Mass Spectrom. 38, 386-393 (2003)... 

Fig. 44.—Enantiomeric determination of monosaccharides by use of anti-carbohydrate antibodies. Plate A = Glc, plate B = Fuc, plate C = Gal, plate D = polysaccharide. (Reprinted from Carbohydrate Polymers, Volume 24, J. H. Pazur, A. J. Reed, and N.-Q. Li, pp. 171-175, copyright 1994, with permission from Elsevier Science Ltd., The Boulevard, Langford Lane, Kidlington, 0X5 1GB, UK.)...

Eljarrat, E., Guerra, R, Barcelo, D. (2008) Enantiomeric determination of chiral persistent organic pollutants and their metabolites. Trends Analyt. C/zew., 27,847-861. 

Yokoyama, L Mizuki, Y. Yamagudii, T. Pfrjii, T. Simultaneous enantiomeric determination of a gas-troprokinetic agent mosapride citrate and its metabolite in plasma using ai-acid glycoprotein HPLC column, J.Pharm.BiomedAnal., 1997,15, 1527-1535. 

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