Electrophoresis PAGE

Protein Purity Is Assessed by Polyacrylamide Gel Electrophoresis (PAGE)... 

Infrared spectroscopy, including FTIR Polyacrylamide gel electrophoresis (PAGE) Amplified ribosomal DNA restriction analysis (ARDRA)... 

The total proteins synthesized by bacteria grown under standard conditions, when analyzed by polyacrylamide gel electrophoresis (PAGE), form patterns that can be compared to those of known strains by visual or computer-assisted... 

Urinary proteins were analyzed by SDS-polyacrylamide gel electrophoresis (PAGE), and a 70-kDa protein was identified as the major component of cat urine (Fig. 4.1 A). Comparative analysis of urinary proteins in several other mammals such as humans, mice, dogs, and cattle did not detect a 70-kDa protein. Therefore, the 70-kDa protein was purified from cat urine and characterized by biochemical methods (Miyazaki, Kamiie, Soeta, Taira and Yamashita 2003). Analysis of tissue distribution indicated that the 70-kDa protein is expressed in the kidney in a tissue-specific manner and secreted from the proximal straight tubular cells of the kidney into the urine (Fig. 4.IB). A full-length cDNA for a 70-kDa protein was cloned from a cat kidney cDNA library. The cDNA clone encoded a polypeptide of 545 amino acid residues. The deduced amino acid sequence shared 47% identity with cat carboxylesterase (CES, EC 3.1.1.1), and contained both the CES family protein motif (EDCLY) and a conserved active site motif (GESAG) associated with... 

Typical mass spectroscopy, AFM and gel electrophoresis (PAGE) results are shown in Table 27.2 for structures in the core-shell series [X] (Y)(Z) , where [X] = core, (Y) = shell, (Z) = capping groups and n = number of shell-type dendrimers bonded to the core (Table 27.2). 

Molecular weights for the final products were determined by MALDI-TOF-MS or (polyacrylamide) gel electrophoresis (PAGE). They were corroborated by calculated values from AFM dimension data and were found to be in relatively good agreement within this series (Table 27.2). Calculations based on these experimentally determined molecular weights allowed the estimation of shell filling levels for respective core-shell structures within this series. A comparison with mathematically predicted shell saturated values reported earlier [34], indicates these core-shell structures are only partially filled (i.e. 40-66% of fully saturated shell values, see Table 27.2). 

DEAE is the abbreviation for DiEthyl Amino Ethyl, the binding group of a substitution on cellulose. SDS/PAGE is a technique for naming the material used for electophoresis in which SDS represents sodium dodecyl sulfate solution that is used with PolyAcrylimide Gel Electrophoresis (PAGE). 

SEC purified -D-g ucosidase. This enzyme grade was prepared by applying diafiltered SP188 to the Sephacryl S-200 column. The column was operated at a flow rate of 15 mL/min in 10 mM phosphate buffer pH 6.5 with 100 mM NaQ. -D-glucosidase activity was found in an early eluting peak which proved to be approximately 92% -D-glucosidase by SDS-polyacrylamide gel electrophoresis (PAGE)(data not shown). 

Polyacrylamide gel electrophoresis (PAGE) and cellulose acetate membrane electrophoresis (CAME) were applied to distinguish escolar and oilfish from 27 commercial fish based on muscle protein differences (Ochiai et al., 1984). Myogen fractions from the muscles were subjected... 

Surfactants are amphophilic molecules, which consist of a hydrophobic carbohydrate part and a hydrophilic head group. In capillary zone electrophoresis (CZE), different types, i.e., anionic, cationic, but also neutral, tensides are employed. The ability of such molecules to interact with ionic and nonionic species has been used in ion chromatography and, in particular, in SDS-poly-(acrylamide) gel electrophoresis (PAGE) (15). 

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