Capillary Polyacrylamide Gel Electrophoresis C-PAGE

Polyacrylamide gels have been prepared inside capillaries, and CE separations performed with these capillaries show that electroosmosis has been eliminated, and 

The dideoxy DNA sequencing method begins with the denaturation of double-stranded DNA (dsDNA) into single-stranded DNA. The ssDNA is then annealed with a fluorescent dye-labeled primer, which is an oligodeoxynucleotide 20 bases long. The heteroduplex formed is then incubated in four separate reactions. 

Each reaction mixture contains the heteroduplex, DNA polymerase, a mixture of the four dNTP species (N = G, A, T, or C), and a small amount of one of the four dideoxynucleotide triphosphates (ddNTP), where no 3 -OH group is present. The ratio of dNTP/ddNTP is high, about 1200 1. DNA polymerase sequentially extends the primer with bases complementary to those present on the opposite strand, but stops when one of the ddNTP species is incorporated. This yields a mixture of fragment lengths, all labeled, where polymerization has stopped at one 

---