EDTA Versene

Treatment with chelating agents such as EDTA which removes divalent ions, leads to dissociation of cell monolayers and release of the cells into suspension without protease action. Perfusion of rat liver with citrate solutions has also been used in attempts to produce primary liver cell cultures. [Pg.61]

Very often, however, mixtures of trypsin or trypsin-citrate with versene are used to release cells from monolayer cultures as the combination works very much faster than either alone. In our laboratory the following procedure is used to make up 10 1 of versene solution in PBS-A [Pg.61]

For many biochemical studies it is undesirable to release the cells from the substratum using trypsin. This is especially true in two circumstances. [Pg.62]

1) Studies on cellular surfaces. Trypsin leads to loss of surface proteins including glycoproteins and other antigens and it is these changes which presumably lead to cell death when trypsinisation is prolonged. [Pg.62]

2) Studies involving timed incubations with drugs, radioactive precursors, hormones, etc. In these instances it is important to harvest the cells promptly without exposure at 37°C to an altered environment for an indefinite period. [Pg.62]

The sum of calcium and magnesium ions in the mud determines the total hardness. These ions are analyzed with complexometric titrations using EDTA (versenate). [Pg.33]

EDTA (versene) K 2.00 Acetic acid 4.73 Phosphoric acid K2 6.70 Ammonium hydroxide 9.30... [Pg.21]

EDTA (versene) K2 2.67 Malic acid K2 5.05 2-Aminopurine 7.14 Glycine K2 9.60... [Pg.21]

Phthalic acid Ki 2.90 Succinic acid K2 5.60 4- or 5-Methylimidazole 7.52 EDTA (versene) 10.26... [Pg.21]

If AAS is not possible, determine Ca and Mg by EDTA/versenate method as described previously. (No pretreatment with HCl-HNOg is required). [Pg.124]

Leckie WJH, Tomsett SL. The diagnostic and therapeutic use of edathamil calcium disodium (EDTA Versene) in excessive inorganic lead absorption. Q J Med 1958 27 65-82. [Pg.505]

Versene 100 EP, Versene 100 LS, Versene 100 SRG, Versene 100 XL, Versene 100 Versene 220. See Tetrasodium EDTA Versene Acid. See Edetic acid Versene CA. See Calcium disodium EDTA Versene Diammonium EDTA. See Diammonium EDTA... [Pg.4671]

Versene EDG. See Disodium ethanoldiglycine Versene NA, Versene Ns2. See Disodium EDTA Versene Photo Chelate. See Ferric ammonium EDTA... [Pg.4671]

ETHYLENEDIAMINE TETRACETIC ACID (EDTA, VERSENE, SEQUESTRENE)... [Pg.131]

Parenteral 100 mg/mL for IM injection Edetate calcium [calcium EDTA] (Calcium Disodium Versenate)... [Pg.1244]

RAW MATERIALS Versene 100/220 CHEMICAL DESCRIPTION Solution of tetrasodium salt of EDTA SOURCE Dow... [Pg.196]

Edathamil EDTA Havidote, Versene acid. Titriplex II... [Pg.60]

EC10 EDTA Eagle s MEM supplemented with 10% calf serum ethylenediaminetetraacetic acid (versene)... [Pg.370]

EDTA Ethylenediaminetetraacetic acid, Versene, (ethylenedinitrilo)tetraacetic acid... [Pg.7]

Dissolvine edathamil EDTA ethylenediaminetetraacetic acid (ethylenedinitrilo)tetraacetic acid Sequestrene AA tetracemic acid Versene Acid. [Pg.260]

Synonyms edetate sodium edetic acid tetrasodium salt EDTA tetrasodium N,N -l,2-ethanediylbis[N-(carboxymethyl)-glycine] tetrasodium salt ethylenebis(iminodiacetic acid) tetrasodium salt ethylenediaminetetraacetic acid tetrasodium salt (ethylenedinitrilo)tetraacetic acid tetrasodium salt Sequestrene NA4 tetracemate tetrasodium tetracemin tetrasodium edetate tetrasodium ethylenebis(iminodi-acetate) tetrasodium ethylenediaminetetraacetate Versene. [Pg.262]

Synonyms edetate trisodium edetic acid trisodium salt EDTA trisodium N,N -1,2-ethanediylbis[N-(carboxymethyl)gly-cine] trisodium salt ethylenediaminetetraacetic acid trisodium salt (ethylenedinitrilo)tetraacetic acid trisodium salt Sequestrene NA3 trisodium ethylenediaminetetraacetate Versene-9. [Pg.262]

Versene 1 mM disodium ethylenediaminetetraacetic acid (EDTA), 0.5% phenol red. [Pg.145]

Uarboxymethyt)glycine trisodiunt salt iethyleriedinitrilo tet-raacetic acid trisodium salt EDTA trisodium ethylenediaminetetraacetic acid trisodhnn salt trisodium ethylenediaminetetraacetate trisodium edetate edetic acid trisodiutn salt Limclair Versene-9 Sequestrene NA 3. C1 H O, mol wt 358.20. C 33.53%, H 3.66%, N 7.82%, Na 19.25%, O 35.73%. Prepn Bersworth, U,S. pat. 2,461,519 (1949). Review Biermans, Henrard, Ind. Chim. 39, 6 (1952). [Pg.550]

AI3-18050 CCRIS 294 Edetate Trisodium EDTA EDTA trisodium salt EINECS 205-758-8 Limclair NCI-C03974 Nevanaid-B powder Perma kleer 50, trisodium salt Sequestrene Na3 Sequestrene NA3 Sequestrene trisodium Sequestrene trisodium salt Trilon AO Trisodium edetate Trisodium EDTA Trisodium ethylenediaminetetraacetate Trisodium ethylenediamine-tetraacetate trihydrate Trisodium versenate Versene-9. Chelating... [Pg.258]

AI3-18049 CBC 50152966 CCRIS 3658 Cheladrate Chelaplex III Chelaton 3 Chelaton III Chelest 200 Chelest B Clewat N Compiexon III Dinatrium ethylendiamintetraacetat Diso-Tate Disodium edathamil Disodium edetate Disodium edta, anhydrous Disodium EDTA Disodium salt of EDTA Disodium sequestrene Disodium tetracemate Disodium versenate Disodium versene Dotite 2NA DR-16133 E.D.T.A. disodique Edathamil disodium ... [Pg.270]

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