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DNases enzymology

Demple, B., and Harrison, L. (1994). Repair of oxidative damage to DNA Enzymology and biology. Anna Rev Biochem 63, 915-948. [Pg.435]

Wn, R., 1993. Development of enzyme-ba.sed mediods for DNA. sequence analy.sis and dieir application in genome projects. Methods in Enzymology 67 431-468. [Pg.392]

Maxam, A.M. and Gilbert, W. (1980) Sequencing end-labeled DNA with base-specific chemical cleavages. Methods in Enzymology 65, 499-560. [Pg.85]

Dickerson RE (1992) In Lilley DM, Dahlberg JE (eds) Methods in enzymology, vol 112 DNA structures. Part B. Chemical and electrophoretic analysis of DNA, Academic Press, New York... [Pg.72]

Selected entries from Methods in Enzymology [vol, page(s)] Analysis of GTP-binding/GTPase cycle of G protein, 237, 411-412 applications, 240, 216-217, 247 246, 301-302 [diffusion rates, 246, 303 distance of closest approach, 246, 303 DNA (Holliday junctions, 246, 325-326 hybridization, 246, 324 structure, 246, 322-324) dye development, 246, 303, 328 reaction kinetics, 246, 18, 302-303, 322] computer programs for testing, 240, 243-247 conformational distribution determination, 240, 247-253 decay evaluation [donor fluorescence decay, 240, 230-234, 249-250, 252 exponential approximation of exact theoretical decay, 240, 222-229 linked systems, 240, 234-237, 249-253 randomly distributed fluorophores, 240, 237-243] diffusion coefficient determination, 240, 248, 250-251 diffusion-enhanced FRET, 246, 326-328 distance measurement [accuracy, 246, 330 effect of dye orientation, 246, 305, 312-313 limitations, 246,... [Pg.290]

Selected entries from Methods in Enzymology [vol, page(s)] Biomolecular vibrational spectroscopy, 246, 377 Raman spectroscopy of DNA and proteins, 246, 389 resonance Raman spectroscopy of metalloproteins, 246, 416 structure and dynamics of transient species using time-resolved resonance Raman spectroscopy, 246, 460 infrared spectroscopy applied to biochemical and biological problems, 246, 501 resonance Raman spectroscopy of quinoproteins, 258, 132. [Pg.698]

One area of basic biochemical research that has paid unexpected dividends is DNA replication. Enzymological work here has characterized the various DNA polymerases in bacterial and eukaryotic cells. With progress in the biochemical characterization of these enzymes, new applications have been found for them in research... [Pg.40]

Early basic research on viral infections of E. coli led to the discovery of enzyme systems that protect the bacterium against viral infection. These restriction systems, so-called because they restrict the growth of the virus, were found to be of two general types, differing in their enzymology. Type II systems are now used in recombinant DNA work. [Pg.46]

To explain the enzymology of DNA replication, we first introduce the enzymes that degrade DNA rather than synthesize it. These enzymes are known as nucleases, or DNases if they are specific for DNA rather than RNA. Every cell contains several different nucleases, belonging to two broad classes exonucleases and endonucleases. Exonucleases degrade nucleic acids from one... [Pg.952]

Source Adapted from T. A. Baker and S. H. Wickner, Genetics and enzymology of DNA replication in Escherichia coli. Ann. Rev. Genetics, 26 447, 1992. [Pg.655]

From the complementary duplex structure of DNA described in chapter 25, it is a short intuitive hop to a model for replication that satisfies the requirement for one round of DNA duplication for every cell division. In chapter 26, DNA Replication, Repair, and Recombination, key experiments demonstrating the semiconservative mode of replication in vivo are presented. This is followed by a detailed examination of the enzymology of replication, first for how it occurs in bacteria and then for how it occurs in animal cells. Also included in this chapter are select aspects of the metabolism of DNA repair and recombination. The novel process of DNA synthesis using RNA-directed DNA polymerases is also considered. First discovered as part of the mechanisms for the replication of nucleic acids in certain RNA viruses, this mode of DNA synthesis is now recognized as occurring in the cell for certain movable genetic segments and as the means whereby the ends of linear chromosomes in eukaryotes are synthesized. [Pg.993]

The pace of discovery also increased in enzymology, particularly in studies of cellular metabolism. The creation of new substances that could produce other substances made possible the discoveries in DNA and genetic research that followed. [Pg.71]

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See also in sourсe #XX -- [ Pg.8 , Pg.9 , Pg.10 , Pg.11 , Pg.12 , Pg.13 , Pg.14 ]



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