DNA/RNA synthesis

This biotechnology company has been a partner to researchers engaged in the field of DNA/RNA synthesis by starting as a supplier of high purity products and evolving to an active contract researcher. ChemGenes Corporation has developed its expertise in the field of DNA/RNA synthesis. Both, the reagent products and the specialized custom services are provided, either as services or as a research contractor. 

To DNA-RNA synthesis through ribose 5-phosphate. NADPH inhibits. 

Function To provide purines (A and G) for energy metabolism and for DNA-RNA synthesis. 

Incorporation of false building blocks (3). Unnatural nucleobases (6-mercaptopurine 5-fluorouracil) or nucleosides with incorrect sugars (cytara-bine) act as antimetabolites. They inhibit DNA/RNA synthesis or lead to synthesis of missense nucleic acids. 

The discovery of DNA polymerase and its dependence on a DNA template led to the search for enzymes which could make an RNA molecule complementary to the DNA. RNA synthesis does not require a primer strand it does, however, require a specific initiation signal on the DNA template strand to allow binding and initiation. As the RNA strand is synthesised it forms a temporary helix with the template DNA, but when complete the mRNA breaks off at the stop site on DNA. Once released from DNA, some of the RNA is processed further, for the specific structures of rRNA and tRNA. 

Wf Drugs That Inhibit Bacterial DNA/RNA Synthesis and Function... 

DRUGS THAT INHIBIT BACTERIAL DNA/RNA SYNTHESIS AND FUNCTION... 

A normal adult has a total body phosphorus content of 700-800 g [1]. The majority of phosphate is present in bone, although approximately 15% is distributed outside of the skeleton where it is present in the form of inorganic phosphate in extra-cellular fluid and organic phosphates within cells, such as adenosine triphosphate (ATP), nucleic acids, and membrane phospholipids. As such, phosphorus plays a vital role in numerous cell processes including cell energetics, cell membrane formation, and DNA RNA synthesis, to name a few. Within blood, phosphate exists mainly in two forms, HPO and HjPO. These two anions are important serum buffers and their relative concentrations are determined by the serum pH. 

In summary, NTs are drug transporters and key players in those chemotherapeutical approaches that are based on interference with nucleoside salvage and DNA/RNA synthesis (the basis for anticancer therapy), but they themselves are drug targets. They not only modulate extracellular adenosine levels but can also exert other unexpected actions, such as the control of intracellular energy metabolism via AMPK. 

Hydroxychloroquine sulfate is a 4-aminoquinoline compound that interferes with parasitic nucleoprotein (DNA/ RNA) synthesis and parasite growth or causes lysis of parasite or infected erythrocytes. In rheumatoid arthritis, it may suppress formation of antigens responsible for symptom-producing hypersensitivity reactions. It is indicated for prophylaxis and treatment of acute attacks of malaria caused by Plasmodium vivax, Plasmodium malariae, and Plasmodium ovale, and susceptible strains of Plasmodium falciparum. It is also used for treatment of chronic discoid and systemic lupus erythematosus (SLE) and acute or chronic rheumatoid arthritis in patients not responding to other therapies. 

Another family of base protecting groups that has found some favor for DNA-RNA synthesis comprises the 2-(4-nitrophenyl), 5 (Npe), 2-(4-nitro-phenyl)ethoxy carbonyl, 6 (Npeoc), groups [22,23] (Fig. 2). They are stable to both weak acids and bases yet can be readily removed with a nonnucleo-philic base such as l,8-diazabicyclo[5.4.0.]undec-7-ene (DBU). Use of these... 

Figure 6 Linker structures used in solid supports for DNA-RNA synthesis.

Azathioprine (Imuran) Converted to 6-mercaptopurine ribose phosphate which inhibits purine synthesis. Because DNA RNA synthesis requires proteins, all proliferating cells are inhibited (B-cells, T-cells, nonimmune cells). Prevention of organ transplant rejection. Gl distress, bone marrow depression, infections, mild leukopenia and thrombocytopenia. 

A3 32 DNA/RNA synthesis (prop.) Hetero-aromatics Hymexazol Broad spectrum... 

Secondary folate deficiency resulting in megaloblastic anaemia and neuropathy (similar to folate deficiency). This is due to the impaired 1-carbon transfer needed for DNA/RNA synthesis and the impaired methyl transfer needed for synthesis of phosphatidylcholine used in myelin formation. 

Doxorubicin Increases ceramide levels, intercalates with DNA, inhibits DNA/RNA synthesis Dattaefa/., 1995... 

As shown in Table 1.2, metalloenzymes contain specific numbers of metal ions at the active sites in specific proteins. The presence of metal ions allows metalloenzymes to perform functions as biocatalysts for specific enzymatic reactions including gene (DNA, RNA) synthesis, metabolism, antioxidation and so forth. 

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