Nucleotides phosphate

Nicotinamide adenine dinucleotide phosphate reduced tetrasodium salt (reduced diphosphopyridine nucleotide phosphate sodium salt, NADPH) [2646-71-1] M 833.4, pK as for NADP. Mostly similar to NADH above. 

Figure 7.9 Interconversion of sugar phosphates and sugar nucleotide phosphates. Adapted from "Biotechnology of microbial exopolysaccharides". IW Sutherland, Cambridge University Press, 1990.

The genes responsible for encoding the proteins specifically involved in exopolysaccharide synthesis are dustered in one large operon. The genes encoding the proteins for sugar nucleotide phosphates, which are not necessarily spedfically used for exopolysaccharide synthesis, also tend to be dustered. 

Adenine nucleotides Phosphate (P7) Dicarboxylates/P " exchange 2-Oxoglutarate/malate exchange Pyruvate... 

The molar concentration of the free (Cf) and the bound (Cb) molecules and r could be evaluated from the following equations where D and P represent the total input hgand and the DNA nucleotide phosphate molar concentrations, respectively ... 

Poly(dG-dC) poly(dG-dC) and its methylated analogue structures assume left-handed conformation (Z-DNA) in high molar sodium salt (Na", K" ), in low molar divalent cations (Ca", Mg", Ni ), micromolar concentrations of hexaamine cobalt chloride (Co(NH3)6)Cl3 and in millimolar concentrations of polyamines. In order to analyse the binding of berberine to Z-form DNA, Kumar et al. [186] reported that the Z-DNA structure of poly(dG-dC) poly(dG-dC) prepared in either a high salt concentration (4.0 M) or in 40 mM (Co(NH3)6)Cl3 remained invariant in the presence of berberine up to a nucleotide phosphate/alkaloid molar ratio of 0.8 and suggested that berberine neither bormd to Z-form DNA nor converted the Z-DNA to the... 

Fig. 5.10. The formula of one of the mononuclear molybdenum cofactors, Moco. Others have a nucleotide phosphate extension (see references to these elements in Further Reading). In sulfide-rich environments, tungsten replaced molybdenum. In some coenzymes, two pterins are bound to the metal ions.

Strata- Primary and secondary amine- Weak anion exchange and Nucleotide phosphates (95)... 

Anion-exchange high-pressure liquid chromatography provides an excellent method for the determination of adenosine phosphates.226 Nucleotide phosphates have been concentrated by ion-exchange chromatography.227... 

This pathway is variously known as the pentose phosphate, hexose monophosphate or phosphogluconate pathway, cycle or shunt. Although the pentose phosphate pathway achieves oxidation of glucose, this is not its function, as indicated by the distribution of the pathway in different tissues. Only one of the carbons is released as CO2, the key products are NADPH and ribose 5-phosphate, both of which are important for nucleotide phosphate formation and hence for synthesis of nucleic acids (Chapter 20). The... 

Monosaccharides also form phosphate esters with phosphoric acid. Monosaccharide phosphate esters are important molecules in biological system. For example, in the DNA and RNA nucleotides, phosphate esters of 2-deoxyribose and ribose are present, respectively. Adenosine triphosphate (ATP), the triphosphate ester at C-5 of ribose in adenosine, is found extensively in living systems. 

Pi bonds are more reactive than sigma bonds. Carbon, nitrogen, oxygen and sulfur are the only atoms that commonly form re-bonds. Phosphorous forms tc-bonds with oxygen in nucleotide phosphates such asATR... 

Aluminum can form complexes with many molecules in the body (organic acids, amino acids, nucleotides, phosphates, carbohydrates, macromolecules). Free aluminum ions (e.g., A1(H20)63+) occur in very low concentrations. The toxicokinetics of aluminum can vary, depending on the nature of these complexes. For example, aluminum bound in a low-molecular-weight complex could be filtered at the renal glomeruli and excreted, while aluminum in a high-molecular-weight complex would not. 

C. B. Ould-Moulaye, C. G. Dussap, nd J. B. Gros. A consistent set of formation properties of nucleic acid compounds nucleosides, nucleotides, and nucleotide phosphates in aqueous solution, Ther-mochimica Ala, 387, 1 15 (2002). 

The nature of binding of nucleotide phosphates to metal-exchanged bentonite clays as occurring via a single oxygen linkage was established by 31P n.m.r.102... 

A tetraamidinium functionalized, bowl-type cavitand (receptor 8) was developed by Diederich and Sebo [47]. This receptor was found to complex 1,3-dicarboxylate anions with good selectivity and a 1 2 binding stoichiometry both in CD3OD and D20, as revealed by standard Job plot analysis. In contrast, various nucleotide phosphates were found to be bound with a 1 1 stoichiometry in D20. In the case of the adenosine phosphates, the association constants increased as a function of nucleotide charge [i.e., the affinity order (K., M-1) was cAMP (1400) < AMP (10000)charged receptor (8) also showed moderate selectivity towards AMP (Ka = 10 000 M-1) over other nucleotide monophosphate anions, such as GMP (FCa = 5200 M-1), CMP (fCa = 3500 M-1), TMP (K l — 5900 M ), and UMP (Ka-3800M ) in D20 containing TRIS buffer (2.5 mM, pH 8.3). 

The most generally interesting intracellular metabolites are the adenine nucleotide phosphates (ATP/ADP/AMP) and the nicotinic acid/adenine dinucleotide phosphates (NAD(P)H/NAD(P)). The pool concentrations and ratios of these can serve as markers for the physiological status of a culture. 

One of the simplest methods of estimation of PolyPs in extracts is based on the assay of Pi, which is released from the PolyPs by hydrolysis with 1 M HC1 at 90 °C for 10 min. The Pi released under these conditions is defined as labile phosphorus . If the compounds containing organic labile phosphorus (i.e. nucleotide phosphates, sugar phosphates, etc.) were removed from the extracts by adsorption on Norit charcoal, the increase in Pj content after hydrolysis can be attributed to PolyP and pyrophosphate (PPi). Estimation of the PPj content (Mansurova, 1989) before hydrolysis may be needed in some cases for more precise calculations of the PolyP content. Pi may be determined by one of the well-known chemical methods (Fiske and Subarrow, 1925 Weil-Malerbe and Green, 1951). 

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