Incubation conditions for

The incubation conditions for trypsin and pepsin were chosen after preliminary tests showed that measurable amounts of collagen were released into the incubation solutions. In trypsin incubations, degradation is fastest on day 1 and decreasing in velocity thereafter. Slices digested extensively by pepsin were fragile. Infermittent changes of pepsin solutions were therefore avoided. 

Every 4 months, open a new ampoule and subculture to TSB. The incubation conditions for this organism are 24 hours at 32 2°C. Subculture from the TSB to (TSA) slopes (stock slopes) and concurrently plate onto a TSA plate to check for purity. 

Table 6 Typical In Vitro Incubation Conditions for CYP Inhibition Studies in Human Liver Microsomes and Selected Analytical and Kinetic Parameters... 

Incubation Conditions for Golgi-Membrane Binding and Vesicle Budding Reactions... 

The precise reaction conditions are flexible. The protocol described here is based on that supplied with the Superscript II reverse transcriptase In earlier studies (14) using 15 U AMV reverse transcriptase (Promega, Southampton, UK) we used 2 5-fold lower concentrations of dNTPs and one-third the amount of oligo d(T) primer, in reactions containing 2.5 U RNasin and 5 mM MgCl2 together with reaction buffer supplied with the enzyme Incubation conditions for this enzyme were 15 mm at 42°C. 

Media and Incubation Conditions for the Detection of Heterotrophic Flora... 

TABLE I. Reacticm Mixtures and Incubation Conditions for Glycosyltransferase Assays"... 

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