Distillation/extraction wheat plant extracts

Comparison of fresh and frozen immature plants. To obtain an adequate amount of field grown wheat for initial identification of compounds, plants were harvested and stored frozen prior to distillation-extraction. After identifications were confirmed a comparison was made between the volatiles from fresh and frozen tissue to determine if the compounds isolated from frozen samples were also present in fresh wheat tissue. For these comparisons immature plants (Figure 1) 20-30cm tall were harvested and macerated in a Waring blender prior to distillation-extraction. From Table II it can be seen that the... 

Over 50 different pyridazin-3-ones were evaluated for biological activity in a wheat (Triticum aestivum L.) test system described previously (1). Briefly, seeds were germinated in 9-cm petri dishes on three layers of filter paper. Pyridazinones were dissolved in acetone and the filter papers were impregnated with 1 ml of acetone solution. After the soluent evaporated, 10 ml of distilled water were added to form an inhibitor concentration of 100 yM. Seeds were planted directly on the moist papers and germinated for 4 days in a controlled environment chamber on a 16-hr photoperiod with 27+lC day temperature and 21+lC night temperature. Light intensity from both fluorescent and incandescent bulbs was 28 klux at dish level. Lipids were extracted and recovered from 1 g of lyophilized shoot tissue, separated into membrane and non-membrane lipids, and analyzed by gas chromatography as described (1). 

Fatty acids as precursors of volatiles in steam distillate-hexane extracts of tobacco and wheat Relative abundances of specific fatty acids in hydrolysates of 2 varieties of burley tobacco stalk and 1 variety of wheat (whole plant Including leaves and stems) are given in Table III. 

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