Peptide Display

Pasqualini, R., and Ruoslahti, E. (1996). Organ targeting in vivo using phage display peptide libraries. Nature 380, 364-366. 

Naik, R.R., Brott, L.L., Clarson, S.J. and, Stone, M.O. (2002) Silica-precipitating peptides isolated from a combinatorial phage display peptide library. Journal of Nanoscience and Nanotechnology, 2, 95-100. 

MAbs directed against the C. neoformans polysaccharide are closely related, and some differ only in a few amino acid residues [142,143]. This fine specificity is related to their protective efficacy. A highly protective IgGl mAh was used to screen phage-displayed peptide libraries, and several binding peptide sequences were identified [144]. The dodecapeptide GLQYTPSWMLVG bound to the mAh with a Kd of 295 nM, measured by surface plasmon resonance (SPR) and inhibited binding of the mAh to the polysaccharide. The dodecapeptide was able to induce only a weak antipolysaccharide antibody response [145]. 

Peptide libraries Intermediaries for non peptide drugs Mirror image phage display Peptides with effector functions Select binders on unknown antigens From 2D gels From expression cDNA libraries... 

Phage displayed peptide libraries have been snccessfnlly employed for the identification of peptide ligands with a variety of applications. Recent reviews describe the developments in the generation and screening of peptide libraries [6], their application in the identification of receptor ligands [32] and in drng development [33]. 

Peptide Receptor Ligands from Phage Display Peptide Library. 269... 

There has been substantial progress in the construction of phage-displayed peptide libraries and in screening methods with the libraries to isolate peptide receptor ligands [66,116-119]. Phage display peptide libraries offer opportunities to characterize peptide binding specificity of important proteins, such as... 

Table 4. Selected examples of short peptide motifs derived from phage display peptide libraries (adapted from [116]) ...

Table 4 lists some examples of proteins that bind short peptide motifs derived from phage display peptide libraries [66,116-121]. Of particular interest is the use of phage display peptide libraries for screening peptides that bind to integrins. Each of the integrins and has a slightly different... 

Another form of displayed peptide library is the FliTrx Random Peptide Display Library (Invitrogen, Paisley, UK), which uses the bacterial flagellum to display random peptide libraries on the E. coli cell surface (14). This library was constructed in the pFliTrx vector, which positions the random peptides in a flagellin (Fli) thioredoxin (Trx) fusion protein. Biopanning with bacteria works surprisingly well in our experience (15) and screening on nitrocellulose is similar to the Smith method. 

Pereboeva, L. A., Pereboev, A. V., and Morris, G. E. (1998) Identification of antigenic sites on three hepatitis C virus proteins using phage-displayed peptide libraries. J. Med. Virol. 56,105-111. 

Epitope mapping using phage display peptides... 

In an approach similar to the cell-like compartments, Doi and Yanagawa (1999) used biotinylated DNA to display peptides fused to streptavidin in compartments of water in oil emulsions. The method was named streptavidin-biotin linkage in emulsions, STABLE (Doi and Yanagawa, 1999). Upon in vitro translation each translated peptide is displayed as a fusion to streptavidin that binds to its encoding biotinylated DNA in its compartment. The resulting protein-DNA fusions can then be recovered and used for affinity selection. To avoid cross-contamination, biotin has to be added before recovery because much more streptavidin will be produced in each compartment than biotinylated DNA is present. The selected DNA-protein complexes can then be amplified by PCR. The principle of this selection strategy is shown in Figure 7. 

Because the peptide sequences are very short in such systems, few (if any) errors are introduced by the polymerase and even error-prone PCR techniques are not ideal for introducing sequence changes. Therefore, unless a cassette mutagenesis strategy is followed, the benefit of ribosome display for an evolutionary approach is not exploited with displayed peptides. What is exploited, however, is the possibility to use larger libraries than those possible with in vivo selection systems. 

Examples of display peptide library screening on specific targets... 

The library is allowed to react with biotinylated target in solution, usually a MAB, then added to a streptavidin-coated Petri dish. Phages displaying peptides binding to the antibody are adsorbed to the plastic surface through biotin-streptavidin bonds. 

Tafi R, Bandi R, Prezzi C, Mordelli MU, Cortese R, Monaci P, Nicosia A, Identification of HCV core mimotopes Improved method for the selection and use of disease-related phage-displayed peptides, Biol. Chem., 378 495-502, 1997. 

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