Target biotinylated

An enzyme-amplified detection scheme, based on tire coupling of a streptavidin-alkaline phosphatase conjugate and biotinylated target sequences was then applied. The enzyme catalysed the hydrolysis of the elecn oiiractive a-naphthyl phosphate to a-naphtlrol this product is elecU oactive and has been detected by means of differential... 

Other fluorescent probes also may be used to label (strept)avidin molecules for detection of biotinylated targeting molecules. Chapter 9 reviews many additional fluorescent labels, such as quantum dots, lanthanide chelates, and cyanine dye derivatives, all of which may be used in similar protocols to create detection conjugates for (strept)avidin-biotin-based assays. 

Breast cancer E908X-WT DNA Hybridisation of biotinylated target strand to oligonucleotide probe-coated magnetic beads, then addition of streptavidin-Au particles Dissolution of Au tag, and its chronopotentiometric stripping analysis at a single-use SPCE +5.0gA... 

Lambda-exonuclease treatment. This procedure is used by Alderon Biosciences [28]. Exonucleases are enzymes that hydrolyse the terminal phosphodiester bonds of DNA strands obtaining mononucleosides monophosphate or biphosphate. In this case a 5 -exo-nuclease that hydrolyses the 5 -end phosphodiester is used. In this way, the strands are destroyed. To achieve the total hydrolysis of the sister strand, the target strand must be protected, for example, by a label at its 5 -end. Therefore, the PCR amplification is carried out using one of the primers biotinylated at the 5 -end and the other one modified at its 5 -end with a phosphate group. The extension of these primers give rise to the 5 -biotinylated target DNA strand and a 5 -phosphate sister DNA strand, which is hydrolysed by 5 -exonuclease. 

Deposit 20 pL drops of different concentrations of biotinylated target strand or biotinylated three-base mismatch target for 60 min at room temperature. 

Incubate slide with biotinylated target protein. 

The library is allowed to react with biotinylated target in solution, usually a MAB, then added to a streptavidin-coated Petri dish. Phages displaying peptides binding to the antibody are adsorbed to the plastic surface through biotin-streptavidin bonds. 

FIGURE 19.4 Biopanning selection through biotin-streptavidin interaction. The library is incubated with the biotinylated target in solution phages displaying the active binding peptide are captured on a streptavidin-coated Petri dish, eluted, and amplified. 

DNA is fixed directly on the graphite-epoxy composite (GEC) substrate by dry-adsorption [18-20]. Hybridization was confirmed through biotinylated target DNA and HRP-SA conjugate interaction. It is possible to measure very low concentrations of target DNA with the electrochemical detection method. 

Biotinylated target protein followed by streptavidin-alkaline phosphatase are used to screen bead library positive beads turned dark upon adding of BCIP substrate. 

Ellipsometry quantification and rapid visual detection of DNA hybridization, using biotinylated target sequences, on amorphous sihcon and diamond-like carbon deposited on a porous polycarbonate membrane, creating a gold-coloured reflective test surface coated with immobihzed ssDNA capture probe were described by Ostroif et al. [38]. 

Figure 5 Biopanning on magnetic beads. The binding of the library to biotinylated target in solution is followed by capture of phages on streptavidin-coated magnetic beads and by selection of bound phages through a magnetic field.

The approach using PNA hybridisation probes for MALDI-TOF mass spectrometric analysis is comprised of the following steps (Figure lb) immobilisation of biotinylated target DNA (e.g. a PCR amplicon) by binding to streptavidin coated magnetic beads dissociation and removal of the non-biotinylated... 

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